AM00058PU-N MAPK7 / BMK1 / ERK5 (incl. pos. control) antibody
see related secondary antibodiessee all 22 MAPK7 / BMK1 / ERK5 products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti MAPK7 / BMK1 / ERK5 (incl. pos. control) 12F2
Synonyms
MAP Kinase 7 BMK1 kinase ERK-5 ERK4 PRKM7 Mitogen-activated protein kinase 7, Mitogen-activated protein kinase 7
Product review
Please read our product review about MAPK7 / BMK1 / ERK5 (incl. pos. control): Antibodies to MAP-Kinases.
Product Description
Mouse anti MAPK7 / BMK1 / ERK5 (incl. pos. control) 12F2, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 12F2 |
| Catalog Number | AM00058PU-N |
| Swiss Prot. No. | Q13164 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00058PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to MAPK7/erk5 | |
| Background | Erk5 (also known as MAPK7 or big mitogen activated kinase 1, BMK1) belongs to the Ser/Thr protein kinase family. It contains a T E Y motif in the activation loop similar to Erk1/2. Extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxdative stress lead to Erk5 activation by MEK5. The Erk5 pathway plays an important role in cellular proliferation, differentiation and survival. |
| Immunogen | Peptide conjugated to hemocyanin. Swiss Num.: Q13164 AA Sequence: N-Terminus |
| Format | State: Lyophilized Purification: Size exclusion chromatography BufferSystem: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose Reconstitution: Restore with 1 ml H2O (15 min, RT). |
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive control: Cell lysate from untreated SKOV-3 cells. |
| Specificity | This antibody recognizes MAP kinase 7 (erk5) at 92 kDa in Western-blot applications. Species: Human, Mouse, Rat, Dog. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Pictures | Detection of endogenous MAPK7 / erk5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. Immunoblots were probed with mab MAPK7-12F2 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
