Double click on above image to view full picture
AM00084PU-N ERK1/2 (p42/44 MAP Kinase) pT-E-pY Motif (incl. pos. control) antibody
see related secondary antibodiessee all 3 ERK1/2 (p42/44 MAP Kinase) pT-E-pY Motif (incl. pos. control) products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti ERK1/2 (p42/44 MAP Kinase) pT-E-pY Motif (incl. pos. control) 12D4
Synonyms
ERK-1/2 MAPK1/2 P42/P44-MAPK p38 p40 p41
Product review
Please read our product review about ERK1/2 (p42/44 MAP Kinase) pT-E-pY Motif (incl. pos. control): Antibodies to MAP-Kinases.
Product Description
Mouse anti ERK1/2 (p42/44 MAP Kinase) pT-E-pY Motif (incl. pos. control) 12D4, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Canine ( Can ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 12D4 |
| Catalog Number | AM00084PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00084PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to MAPK/erk // (pT - E - pY) | |
| Background | Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y. |
| Immunogen | Synthetic phosphopeptide conjugated to KLH |
| Format | State: Lyophilized purified IgG Purification: Size exclusion chromatography BufferSystem: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose Reconstitution: Restore with 1 ml H2O (15 min, RT). |
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 µg/ml. Immunoprecipitation: 1 - 10 µg per 10e6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 µg/ml. Immunohistochemistry on frozen sections. |
| Specificity | This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2. Species: Human, Mouse, Dog, Rat. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Pictures | Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK-12D4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
