AM00494PU-N 14-3-3 Protein sigma antibody
see related secondary antibodiessee all 7 14-3-3 Protein sigma products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti 14-3-3 Protein sigma 1433S01
Synonyms
Stratifin SFN HME1 Epithelial Cell Marker Protein 1
Product review
Please read our product review about 14-3-3 Protein sigma: Antibody Panel to Proteins of the 14-3-3 family (14-3-3 beta, 14-3-3 gamma, 14-3-3 epsilon, 14-3-3 zeta, 14-3-3 theta, 14-3-3 sigma, 14-3-3 eta).
Product Description
Mouse anti 14-3-3 Protein sigma 1433S01, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 1433S01 |
| Catalog Number | AM00494PU-N |
| Swiss Prot. No. | P31947 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00494PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to 14.3.3 sigma | |
| Concentration | 0.2 mg/ml |
| Background | 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. |
| Immunogen | Recombinant human 14-3-3 sigma protein Swiss Num.: P31947 |
| Format | State: Liquid ascites fluid Purification: Protein G chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide |
| Applications | Immunofluorescence.
Western Blotting: 1 µg/ml for 2 hrs at RT. Immunoprecipitation: use Protein G, 2 µg/mg protein lysate. Immunohistology (Formalin/paraffin): 2-4 µg/ml for 30 min at RT, staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: HT29 cells. Skin. |
| Specificity | This antibody is highly specific to 14.3.3 sigma and shows no cross-reaction with other isoforms of 14.3.3. Species: Human. |
| Add. Information | Mol. Wt. of Antigen: 26-30kDa
Cellular Localization: Cytoplasmic |
| Storage | 1. Laronga C, et al. J Biol. Chem 2000.
2. Villaret DB, et al. Laryngoscope 2000; 110: 374-81. 3. Hermeking H, et al. Mol Cell 1997; 1(1):3-11. |
| Pictures | Formalin-fixed, paraffin-embedded human skin stained with AM00494PU-N using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00494PU-N 14-3-3 Protein sigma'.
