AM03035PU-N H-Ras antibody
see related secondary antibodiessee all 3 H-Ras products
0.1 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti H-Ras H-Ras-03
Product Description
Mouse anti H-Ras H-Ras-03, Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | H-Ras-03 |
| Catalog Number | AM03035PU-N |
| Quantity | 0.1 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM03035PU-N.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation. |
| Immunogen | Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein. |
| Format | State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Purification: Protein A affinity chromatography. BufferSystem: PBS, pH 7.4 with 15 mM sodium azide as preservative. |
| Applications | ELISA.
Western Blotting under Reducing conditions (0.5-1 μg/ml): Positive control: RAJI human Burkitt lymphoma cell line HeLa human cervix carcinoma cell line K567 human leukemia cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. |
| Specificity | The antibody H-Ras-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid freeze/thaw cycles. Shelf life: One year from despatch. |
| References | 1. Prior IA, Harding A, Yan J, Sluimer J, Parton RG, Hancock J.F.: GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001 Apr;3(4):368-75.
2. Zheng Y, Quilliam L.A: Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO Rep. 2003 May;4(5):463-8. 3. Rotblat B, Prior IA, Muncke C, Parton RG, Kloog Y, Henis YI, Hancock J.F.: Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol Cell Biol. 2004 Aug;24(15):6799-810. 4. Roy S, Plowman S, Rotblat B, Prior IA, Muncke C, Grainger S, Parton RG, Henis YI, Kloog Y, Hancock J.F.: Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling. Mol Cell Biol. 2005 Aug;25(15):6722-33. 5. Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y.: Ras and its signals diffuse through the cell on randomly moving nanoparticles. Cancer Res. 2006 Feb 15;66(4):1974-81. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM03035PU-N H-Ras'.
