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Western blot analysis of Erk1/2 expression in PC12 cells.

 

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AP00033PU-N  ERK1/2 (p42/44 MAP Kinase) antibody

see related secondary antibodies
see all 36 ERK1/2 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti ERK1/2 (p42/44 MAP Kinase)

Synonyms

ERK-1/2, MAPK1/2, P42/P44-MAPK, p38, p40, p41, , Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3

Product review
Please read our product review about ERK1/2 (p42/44 MAP Kinase): Antibodies to MAP-Kinases.

Product Description

Rabbit anti ERK1/2 (p42/44 MAP Kinase) , Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )

Properties

ApplicationsImmunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
ReactivityChicken ( Chk ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ), Sheep ( Sh )
PresentationAff - Purified
HostRabbit
Catalog NumberAP00033PU-N
Swiss Prot. No.P27361
Quantity0.1 mg
PriceUS$ 455
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AP00033PU-N.pdf

Datasheet Extract

Polyclonal Antibody to Erk1/2
Alternate namesMAPK
Concentration0.5 mg/ml
BackgroundThe extracellular signal-regulated kinase 1 and 2 (Erk1 and Erk2) are closely related mitogen activated protein (MAP) kinases that are activated through the extracellular stimulation by many growth factors, mitogens and differentiation promoting agents via a protein kinase cascade. Erk1/2 are activated approximately 1000 fold by phosphorylation of neibouring threonine and tyrosine residues by Mek1 and Mek2. Both sites must be phosphorylated for maximum activity. Erk1/2 kinases are ubiquitously distributed in the nervous system, as well as a broad range of cells and tissues. These two kinases represent proximal kinases in the classical MAP kinase cascade pathway which links growth and differentiation signals at the cell surface (through tyrosine kinase) with transcription in the nucleus.
Immunogen
Swiss Num.: P27361
Format
State: Liquid Ig fraction
Purification: Affinity chromatography
BufferSystem: PBS, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal
ApplicationsThe antibody can be used for Western blotting (0.5-4 μg/ml), immunoprecipitation (20-40 μg/ml) and immunohistochemistry (20-40 μg/ml).
SpecificityThe antibody recognizes 42 kDa and 44 kDa of endogeneous Erk1/Erk2 (p44/p42 MAP kinase).
Species: Human, mouse, rat, sheep, chicken, hamster.
StorageStore (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
PicturesWestern blot analysis of Erk1/2 expression in PC12 cells.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'AP00033PU-N ERK1/2 (p42/44 MAP Kinase)'.