AP00511PU-N Catenin beta antibody
see related secondary antibodiessee all 65 Catenin beta products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Catenin beta -
Synonyms
Beta-catenin, Catenin beta-1, CTNNB1, CTNNB, ,
Product review
Please read our product review about Catenin beta: Antibodies to Catenins.
Product Description
Rabbit anti Catenin beta -, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Canine ( Can ), Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00511PU-N |
| Swiss Prot. No. | P35222 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00511PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to Catenin-beta | |
| Concentration | 1 mg/ml |
| Background | The catenins ( alpha, beta and gamma ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. Alpha catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between alpha actinin and alpha catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion. |
| Immunogen | Recombinant human beta catenin protein. Swiss Num.: P35222 |
| Format | State: Liquid IgG fraction Purification: Protein A affinity purified immunoglobulin fraction BufferSystem: 10 mM PBS, pH 7.4, with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Immunofluorescence.
Immunoprecipitation (native): 10 µg/mg protein lysate, use Protein A. Western Blotting (2.5-5 µg/ml for 2 hrs at RT). Immunohistology (Formalin/paraffin: 10-20 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0). Recommended positive control: MCF-7 Cells, Breast Carcinoma. |
| Specificity | Highly specific for Catenin beta.
Cellular Localization: Cytoplasmic, Cell Membrane. Mol. Wt. of Antigen: 92kD. Species: Human and Mouse. |
| Storage | Store at 2-8°C for up to one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1 Rimm D.L., et. al. (1995) Lab Invest 72(5): 2
2. Koslov, E.R., et. al. (1997) J. Biol.Chem. 272(43): 3 3. Hazan, R.B., et. al. (1997) J.Biol.Chem. 272(51):32448-32453 4. Roe, S., et. al. (1998) Cell Adhesion and Commun. 5:283. |
| Pictures | Formalin-fixed, paraffin-embedded human breast carcinoma stained with Catenin beta Ab (Cat. AP00511PU) using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00511PU-N Catenin beta'.