AP00608PU-N Catenin beta antibody
see related secondary antibodiessee all 65 Catenin beta products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Catenin beta -
Synonyms
Beta-catenin Catenin beta-1 CTNNB1 CTNNB
Product review
Please read our product review about Catenin beta: Antibodies to Catenins.
Product Description
Rabbit anti Catenin beta -, Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Chicken ( Chk ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ), Xenopus ( Xen ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00608PU-N |
| Swiss Prot. No. | P35222 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00608PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to Catenin-beta | |
| Concentration | 1.0 mg/ml |
| Background | The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with
E-cadherin at cellular junctions. β- catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion. |
| Immunogen | A synthetic peptide from the C-terminus of the β-catenin protein. Swiss Num.: P35222 Remarks: Mol. Wt. of Antigen: 92 kDa |
| Format | State: Liquid IgG Purification: Protein A chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide. |
| Applications | Immunoprecipitation (Native verified): Use Protein A, Ab 10 µg/mg protein lysate.
Western Blotting: 5-10 µg/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2 µg/ml for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0), for 10-20 min followed by cooling at RT for 20 min.] Positive Control: MCF-7 cells. Breast carcinoma. |
| Specificity | This antibody detects Catenin-beta.
Cellular Localization: Cytoplasmic and cell membrane Species: Human, Mouse, Rat, Chicken and Xenopus. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Rimm D.L., et al(1995) Lab Invest 72(5): 590.
2. Koslov, E.R., et al. (1997) J. Biol.Chem. 272(43): 27301. 3. Hazan, R.B., et al (1997) J.Biol.Chem. 272(51): 32448-32453 |
| Pictures | Formalin-fixed, paraffin-embedded human breast cancer stained with Catenin,beta (AP00608PU-N) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00608PU-N Catenin beta'.
