AP00616PU-N CD340 / ERBB2 antibody
see related secondary antibodiessee all 104 CD340 / ERBB2 products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti CD340 / ERBB2
Synonyms
CD340, ERBB2, HER2, NEU, NGL, EC 2.7.10.1, p185erbB2, C-erbB-2, NEU proto-oncogene, MLN 19, , Tyrosine kinase-type cell surface receptor HER2, Receptor tyrosine-protein kinase erbB-2
Product Description
Rabbit anti CD340 / ERBB2 , Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00616PU-N |
| Swiss Prot. No. | P04626 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00616PU-N.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | c-erbB-2, second member (c-erbB-2/HER-2/neu) of the c-erbB family is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. |
| Immunogen | Synthetic peptide derived from C-terminus of human c-erbB-2/HER-2 protein (see Ref.1) THIS SEQUENCE IS IDENTICAL IN RAT NEU PROTEIN. Swiss Num.: P04626 |
| Format | State: Liquid Purification: Total IgG purified from rabbit antiserum by Protein A chromatography. BufferSystem: 10mM PBS, pH 7.4, with 0.2% BSA & 0.09% azide. |
| Applications | Western Blotting: 5-10 µg/ml for 2hrs at RT. Immunoprecipitation(see Ref.1): use Protein A and Ab at 10 µg/mg protein lysate.
Immunohistology (Formalin/paraffin): 2.5-5.0 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: SKBR-3 or T47D cells or breast carcinomas. |
| Specificity | This polyclonal antibody react equally well with wild and mutant (oncogenic) form c-erbB-2 protein. Molecular weight: 185kDa. Cellular Localization: Cell membrane. Species: Human, Monkey, Rat, and Mouse (see also Ref.1 ). |
| Storage | Store at 2-8°C or in aliquots at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Gullick WJ, et. al. International Journal of Cancer, 1987, 40(2):246-54.
|
| Pictures | Formalin-fixed, paraffin-embedded human breast cancer stained with c-erbB2 Ab (Cat. AP00616) using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00616PU-N CD340 / ERBB2'.
