AP02308PU-S Raf1 (pSer259) antibody
see related secondary antibodiessee all 30 Raf1 products
50 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Raf1 (pSer259)
Synonyms
C-RAF, C-Raf, CRAF,
Product Description
Rabbit anti Raf1 (pSer259) , Presentation: Aff - Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | AP02308PU-S |
| Swiss Prot. No. | P04049 |
| Quantity | 50 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP02308PU-S.pdf |
Datasheet Extract
| Alternate names | C-RAF, C-Raf, CRAF |
| Concentration | 1.0 mg/ml |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-Sp-T-P). Swiss Num.: P04049 |
| Format | State: Liquid purified IgG fraction Purification: Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. BufferSystem: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol. |
| Applications | Western blot (1:500-1:1000).
Immunohistochemistry (1:50-1:100). |
| Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259. Species: Human, Mouse and Rat. |
| Storage | Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
2. Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468. 3. Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283. 4. Chong H, et al. (2001) EMBO J. 20, 3716-3727. 5. King A J, et al. (1998) Nature. 396:180-183. |
| Pictures | Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody AP02308PU |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP02308PU-S Raf1 (pSer259)'.
