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<b>Figure 1.</b>  Western blot analysis of extract from Jurkat cells, using Zap-70  antibody (Line 1 and 2) and Zap-70 (phospho-Tyr319) antibody (AP02441PU, Line 3 and 4).

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AP02441PU-N  ZAP70 (pTyr319) antibody

see related secondary antibodies
100 µg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti ZAP70 (pTyr319)

Synonyms

ZAP-70; ZAP 70; Tyrosine-protein kinase ZAP-70; Syk-related tyrosine kinase; SRK

Product Description

Rabbit anti ZAP70 (pTyr319) , Presentation: Aff - Purified. Product is tested for Westernblot / Immunoblot ( WB )

Properties

ApplicationsWesternblot / Immunoblot ( WB )
ReactivityHuman ( Hu ), Mouse ( Ms )
PresentationAff - Purified
HostRabbit
Catalog NumberAP02441PU-N
Swiss Prot. No.P43403
Quantity100 µg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AP02441PU-N.pdf

Datasheet Extract

Polyclonal Antibody to Zap-70 //(Phospho-Tyr319)
Alternate namesSRK, ZA70
Concentration 1.0 mg/ml
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).
Swiss Num.: P43403
Format
State: Liquid purified Ig fraction.
Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.
BufferSystem: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
ApplicationsSuitable for use in Western blot (1:500~1:1000).
SpecificityThis antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 319.

Species: Human, mouse and rat.
StorageStore the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448.
2. Ku GM, et al. (2001) EMBO J; 20(3): 457-465.
3. Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780.
4. Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774.
5. Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.
PicturesFigure 1. Western blot analysis of extract from Jurkat cells, using Zap-70 antibody (Line 1 and 2) and Zap-70 (phospho-Tyr319) antibody (AP02441PU, Line 3 and 4).

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / 170,00 €
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / 160,00 €
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / 160,00 €
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / 170,00 €
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / 170,00 €
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / 200,00 €
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / 320,00 €
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / 320,00 €
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / 320,00 €
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / 320,00 €
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / 320,00 €
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / 330,00 €
   

Click here to see all secondary antibodies for 'AP02441PU-N ZAP70 (pTyr319)'.