AP03012PU-N CD340 / ERBB2 antibody
see related secondary antibodiessee all 104 CD340 / ERBB2 products
0.05 mg / US$ 275
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti CD340 / ERBB2
Synonyms
CD340, ERBB2, HER2, NEU, NGL, EC 2.7.10.1, p185erbB2, C-erbB-2, NEU proto-oncogene, MLN 19, , Tyrosine kinase-type cell surface receptor HER2, Receptor tyrosine-protein kinase erbB-2
Product Description
Rabbit anti CD340 / ERBB2 , Presentation: Aff - Purified. Product is tested for Paraffin Sections ( P )
Properties
| Applications | Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | AP03012PU-N |
| Swiss Prot. No. | P04626 |
| Quantity | 0.05 mg |
| Price | US$ 275 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP03012PU-N.pdf |
Datasheet Extract
| Alternate names | Neu |
| Concentration | 1.0 mg/ml |
| Background | The oncoprotein ErbB2/HER2 (human epidermal growth factor receptor 2), also known as Neu, is a 185 kDa transmembrane tyrosine kinase of cell surface growth factor receptor family. It is present in a wide variety cell types of normal human fetal and adult tissues and is frequently overexpressed in human carcinomas (e.g. in 20-30% cases of breast cancer cells). Activation of ErbB2 triggers intracellular signalling events, which are essential for cell growth and differentiation. In the last 20 years ErbB2 antigen has become very important marker and therapy target in patient care. |
| Immunogen | Synthetic peptide corresponding to a site on the extracellular domain of the c-ErbB2 oncoprotein (HER2/neu). Swiss Num.: P04626 |
| Format | State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE). Purification: Immunoaffinity chromatography. BufferSystem: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer. |
| Applications | Immunohistochemistry (paraffin sections):
Recommended dilution: 10 μg/ml in PBS with 10% FCS and 0.09 % NaN3 Staining technique: standard ABC technique (DAB+) Pretreatment: Fill the staining dishes with 10 mM citrate buffer pH 6.0 (pretreatment solution). Put the staining dishes with the pretreatment solution in the water bath and heat on 95-99°C. Sink the deparaffin sections into the preheated pretreatment solution. Once again heat the water bath and pretreatment solution with sections on 95-99°C - incubate for 40 min in 95-99°C. Remove the dishes with sections from the water bath and keep them cool down for 20 min in RT. Rinse the sections 3 x 5 min in washing buffer (PBS). Incubation: overnight at 4°C Positive tissue: breast carcinoma Note: Warning: The sections must not drain!!. |
| Specificity | The polyclonal antibody reacts with ErbB2/HER2 oncogen, a 185 kDa transmembrane tyrosine kinase presented in a wide variety cell types of human tissues. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Koeppen HK, Wright BD, Burt AD, Quirke P, McNicol AM, Dybdal NO, Sliwkowski MX, Hillan KJ.: Overexpression of HER2/neu in solid tumours: an immunohistochemical survey. Histopathology. 2001 Feb;38(2):96-104.
2. Dowsett M, Hanna WM, Kockx M, Penault-Llorca F, Rüschoff J, Gutjahr T, Habben K, van de Vijver MJ: Standardization of HER2 testing: results of an international proficiency-testing ring study. Mod Pathol. 2007 May;20(5):584-91. 3. Hicks DG, Kulkarni S: HER2+ breast cancer: review of biologic relevance and optimal use of diagnostic tools. Am J Clin Pathol. 2008 Feb;129(2):263-73. 4. Yuan W, Wang W, Cui B, Su T, Ge Y, Jiang L, Zhou W, Ning G: Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry. Endocr Relat Cancer. 2008 Mar;15(1):343-50. 5. Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Signaling through ShcA is required for TGF-{beta} and Neu/ErbB-2 induced breast cancer cell motility and invasion. Mol Cell Biol. 2008 Mar 10 6. Arora P, Cuevas BD, Russo A, Johnson GL, Trejo J.: Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion. Oncogene. 2008 Mar 31 |
| Pictures | Immunohistochemistry staining of breast carcinoma (paraffin-embedded sections) with polyclonal anti-human ErbB2/HER2 antibody (AP03012PU-N). Primary antibody dilution: Fig. 1A 10 μg/ml, Fig. 1B 5 μg/ml, Fig. 1C 2 μg/ml. Detection by Goat anti-rabbit IgG HRP secondary antibody.
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12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP03012PU-N CD340 / ERBB2'.
