Chicken IgG / Chicken IgY (H+L chain) - AP31795HR-N

Product Description for Chicken IgG / Chicken IgY

Properties: (H+L chain)
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Product Category	Secondary Antibodies
Quantity	0.4 ml
Synonyms	Chicken Immunoglobulin G, Chicken Immunoglobulin Y
Presentation	HRP
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Immunogen	Immunogen: Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Application	Sandwich ELISA: 1/120,000 dilutions of GGHL-30P-2A was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis: Chicken serum (3 ul) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Concentration	1.0 mg/ml (based on absorbance at 280 nm)
Storage	Store the antibody undiluted at 2-8°C in the dark. Shelf life: one year from despatch.
Format	Label: Horseradish Peroxidase HRP Purification: Affinity Chromatography Buffer System: 50mM HEPES, pH 7.0, 0.1M NaCl containing 0.2% BSA as stabilizer and 0.01% Thimerosal as preservative State: Liquid purified IgY fraction
Specificity	Specificity: Chicken IgY (heavy and light chains). Species: Chicken.
Targets	Chicken IgG / Chicken IgY antibody