Product Description for Chicken IgG / Chicken IgY
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for Chicken IgG / Chicken IgY
|Product Category||Secondary Antibodies|
|Synonyms||Chicken Immunoglobulin G, Chicken Immunoglobulin Y|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||Sandwich ELISA: 1/120,000 dilutions of GGHL-30P-2A was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate.
Western blot (1/5000).
Immunoelectrophoresis: Chicken serum (3 ul) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
|Concentration||1.0 mg/ml (based on absorbance at 280 nm)|
|Storage||Store the antibody undiluted at 2-8°C in the dark.
Shelf life: one year from despatch.