BA051 Apolipoprotein A I (APO AI) antigen
see all 45 Apolipoprotein A I products
1 mg / US$ 365
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Apolipoprotein A I (APO AI)
Synonyms
APOA1, ApoA-I, Apo-AI, ApoAI,
Product Description
Human Apolipoprotein A I (APO AI)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | Plasma |
| Catalog Number | BA051 |
| Swiss Prot. No. | P02647 |
| Quantity | 1 mg |
| Price | US$ 365 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA051.pdf |
Datasheet Extract
| Concentration | 2.35 mg/ml (OD280, E0.1% = 1.13) |
| Immunogen | Swiss Num.: P02647 |
| Source | HDL from pooled human plasma |
| Format | State: Purified, liquid Purity: >99 % by SDS-PAGE. The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6 M guanidine hydrochloride - 25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted. BufferSystem: 3 M guanidine hydrochloride, 10 mM Tris, 5 mM DTT, pH 8.0, no preservatives |
| Applications | Apo AI can be renatured into PBS, TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room. |
| Storage | Short term storage at 2-8°C. Long term storage at -20°C.
Storage of dialyzed Apo AI in PBS or TBS with 1 mM EDTA and 0.09 % Sodium Azide should be at 2-8 °C for up to 2 weeks. Storage of Apo AI in PBS or TBS at -20°C may lead to precipitation when thawed. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Caution | All human source materials have tested negative for NHCV/NHIV nucleic acid test, HCV antigen; HBsAg, HBcAg, HIV1Ag; and syphilis. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious. |
| References | 1. Chen, W., et al., (2001), "Preferential ATP-binding Cassette Transporter A1-mediated Cholesterol Efflux from Late Endosomes/Lysosomes", The Journal of Biological Chemistry, 276(47): 43564-43569.
2. Feng, B., et al., (2002), "ABCA1-mediated Cholesterol Efflux Is Defective in Free Cholesterol-loaded Macrophages", The Journal of Biological Chemistry, 277(45): 43271-43280. 3. Gerbod-Giannone, M.C., et al., (2006), "TNF alpha induces ABCA1 through NF-kappaB in macrophages and in phagocytes ingesting apoptotic cells", PNAS, 103(9): 3112-3117. 4. Martinez, L.O., (2003), "Phosphorylation of a Pest Sequence in ABCA1 Promotes Calpain Degradation and Is Reversed by ApoA-I", The Journal of Biological Chemistry, 278(39): 37368-37374. 5. Sun, Y., et al., (2003), "Stearoyl-CoA Desaturase Inhibits ATP-binding Cassette Transporter A1-mediated Cholesterol Efflux and Modulates Membrane Domain Structure", The Journal of Biological Chemistry, 278(8): 5813-5820. 6. Ishiguro, H., et al., (2001), "Retrovirus-mediated Expression of Apolipoprotein A-I in the Macrophage Protects against Atherosclerosis in Vivo", The Journal of Biological Chemistry, 276(39): 36742-36748. |