BA1007 Cytokeratin-8 (KRT8) antigen
see all 60 Cytokeratin-8 products
0.25 mg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Bovine Cytokeratin-8 (KRT8)
Synonyms
KRT-8, CYK8, CYK-8, Cytokeratin8, CK-8, CK8, Keratin-8, Keratin8, K8, K-8, Keratin type II cytoskeletal 8,
Product Description
Bovine Cytokeratin-8 (KRT8)
Properties
| Presentation | Purified |
| Species | Bovine |
| Purity | >95% |
| Source | Liver |
| Molecular Weight | 52 kD |
| Catalog Number | BA1007 |
| Swiss Prot. No. | P05787 |
| Quantity | 0.25 mg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA1007.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Swiss Num.: P05787 |
| Source | Bovine liver |
| Format | State: Lyophilized Purity: > 95% (determined by SDS gelelectrophoresis) BufferSystem: 30 mM Tris/HCI pH 8, 9 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride Reconstitution: Restore with 175 µl distilled water (final volume 250 µl) |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays. Immunization. |
| Description | Bovine keratin K8 (formerly also designated cytokeratin 8) Molecular weight: 52,500 |
| Add. Information | Isoelectric Point: pI 6.4 |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Franke WW, Denk H, Kalt R and Schmid E: Biochemical and immunological identification of cytokeratin proteins present
in hepatocytes and mammalian liver tissue. Exp Cell Res 131, 239 ff (1981) 2. Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 3. Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255 |
| Protocols | Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). |
| Pictures | 1) phosphorylase B (a); BSA (b); ovalbumin (c); carbonic anhydrase (d)
2) keratin K8 |