BA1010 Filamin antigen
see all 32 Filamin products
0.25 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Chicken Filamin
Product Description
Chicken Filamin
Properties
| Presentation | Purified |
| Species | Chicken |
| Purity | >90% |
| Source | Gizzard |
| Molecular Weight | 250 kD |
| Catalog Number | BA1010 |
| Quantity | 0.25 mg |
| Price | US$ 355 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA1010.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (according to Bradford) |
| Source | Chicken gizzard |
| Format | State: Lyophilized Purity: > 90% (determined by SDS gelelectrophoresis) BufferSystem: 20 mM Tris/acetate buffer pH 7.6, 0.1mM EDTA, 2 mM DTT, 20 mM NaCl Reconstitution: Restore with 250 µl Laemmli buffer (final volume 250 µl) or 9M urea buffer (e.g. IEF or NEPHGE buffer) Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step. |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays. Immunization. |
| Description | Filamin, purified polypeptide |
| Add. Information | Isoelectric Point: pI 6.4 |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Feramisco JR and Burridge K: A rapid purification of a-actinin, filamin and a 130,000 dalton protein from smooth muscle. J Biol Chem 255, 1194 ff (1980)
2. Davies PJA, Wallach D, Willingham M, Pastan I and Lewis MS: Self-association of chicken gizzard filamin and heavy merofilamin. Biochem 19, 365 ff (1984) |
| Pictures | 1) filamin
2) myosin (a); Ã?-galactosidase (b); phosphorylase B (c); BSA (d); ovalbumin (e) |