BA1011 Glial fibrillary acidic protein (GFAP) antigen
see all 70 Glial fibrillary acidic protein products
0.25 mg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Bovine Glial fibrillary acidic protein (GFAP)
Synonyms
Glial fibrillary acidic protein, GFAP,
Product Description
Bovine Glial fibrillary acidic protein (GFAP)
Properties
| Presentation | Purified |
| Species | Bovine |
| Purity | >98% |
| Source | Spinal Cord |
| Molecular Weight | 52 kD |
| Catalog Number | BA1011 |
| Swiss Prot. No. | Q28115 |
| Quantity | 0.25 mg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA1011.pdf |
Datasheet Extract
| Immunogen | Swiss Num.: Q28115 |
| Source | Bovine spinal cord |
| Format | State: Lyophilized Purity: > 98% (determined by SDS gelelectrophoresis). BufferSystem: 10 mM sodium phosphate buffer pH 7.5, 6M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride Reconstitution: Reconstitute with 200 µl distilled water (final volume 250 µl) |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays. Immunization. |
| Description | Bovine glial filament protein (GFP) Molecular weight: 52,000 |
| Add. Information | Isoelectric Point: pI 5.4 |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Dahl D, Crosby CJ, Gardner EE and Bignami A: Purification of the Glial Fibrillary Acidic Protein by Anion-Exchange Chromatography. Anal Biochem 126, 165 (1982)
2. Hatzfeld M and Franke WW (1985). J Cell Biol 101, 1826-1841 3. Hatzfeld M et al. (1987). J Mol Biol 197, 237-255 |
| Protocols | Reconstitution to filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). |
| Pictures | 1) myosin (a); Ã?-galactosidase (b); phosphorylase (c): BSA (d); ovalbumin (e)
2) GFP |