BA1016 Cytokeratin-19 (KRT19) antigen
see all 43 Cytokeratin-19 products
0.25 mg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Cytokeratin-19 (KRT19)
Synonyms
KRT-19, Cytokeratin19, CK-19, CK19, Keratin-19, Keratin19, K19, K-19, Keratin type I cytoskeletal 19,
Product Description
Human Cytokeratin-19 (KRT19)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | E. coli |
| Molecular Weight | 44 kD |
| Catalog Number | BA1016 |
| Swiss Prot. No. | P08727 |
| Quantity | 0.25 mg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA1016.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Swiss Num.: P08727 |
| Source | E.coli |
| Format | State: Lyophilized Purity: > 95% (determined by SDS gelelectrophoresis). BufferSystem: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride Reconstitution: Restore with 175 µl distilled water (final volume 250 µl) |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays. Immunization. |
| Description | Recombinant human keratin K19 (formerly also designated cytokeratin 19). Molecular weight: 44 098 (calculated from sequence); 40 000 (determined by SDS gelelectrophoresis) |
| Add. Information | Isoelectric Point: pI 5.1 |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Bader B.L., Jahn L. and Franke W.W.: Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene. Eur. J. Cell Biol. 47, 300-319 (1988).
2. Hofmann I, Franke WW. Heterotypic type I and type II cytokeratins in vitro: viscometry and determinations of relative affinities. Eur J Cell Biol 72:122-132 (1997) 3. Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 4. Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255 |
| Protocols | Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). |