BA1017 Vimentin antigen
see all 73 Vimentin products
0.25 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Vimentin
Synonyms
VIM,
Product Description
Human Vimentin
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | E. coli |
| Molecular Weight | 54 kD |
| Catalog Number | BA1017 |
| Swiss Prot. No. | P08670 |
| Quantity | 0.25 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BA1017.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Swiss Num.: P08670 |
| Source | E.coli |
| Format | State: Lyophilized Purity: > 95% (determined by SDS gelelectrophoresis). BufferSystem: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride Reconstitution: Restore with 175 µl distilled water (final volume 250 µl) |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays. Immunization. |
| Description | Recombinant human vimentin Molecular weight: 53 685 (calculated from sequence); 57 000 (determinated by SDS gelelectrophoresis) |
| Add. Information | Isoelectric Point: pI 5.3 |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Herrmann H, Häner M, Brettl M, Müller SA, Goldie KN, Fedtke B, Lustig A, Franke WW and U Aebi. Structure and Assembly Properties of the Intermediate Filament Protein Vimentin: The Role of its Head, Rod and Tail Domains. J Mol Biol 264, 933-953 (1996)
2. Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 3. Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255 |
| Protocols | Reconstitution to filaments: after vimentin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). |