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BA254  Galactosidase beta antigen


see all 68 Galactosidase beta products
2.5 kU / US$ 414
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

E. coli Galactosidase beta

Synonyms

Beta-Galactosidase, Lactase, lacZ, Beta Galactosidase,

Product Description

E. coli Galactosidase beta

Properties

SpeciesE. coli
Purity600U/mg
Catalog NumberBA254
Swiss Prot. No.P00722
Quantity2.5 kU
PriceUS$ 414
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
BA254.pdf

Datasheet Extract

BackgroundBeta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Immunogen
Swiss Num.: P00722
SourceE.coli
Format
State: Lyophilized
Reconstitution: Restore with sterile distilled water or a dilute buffer of choice.
ApplicationsFunctional Assays.
DescriptionPurified Beta-Galactosidase from E.coli.
Specific Activity: >600 U/mg.
Molecular weight: 540 kD
Add. InformationOne unit hydrolyses 1 µmole of o-nitrophenol-b-D-galactoside to o-nitrophenol per minute at 37ºC, pH 4.5.
StoragePrior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. CAS number 9031-11-2.
2. EC 3.2.1.23.