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BM2274 Ep-CAM / TACSTD1 / CD326 antibody
see related secondary antibodiessee all 25 Ep-CAM / TACSTD1 / CD326 products
50 µg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Ep-CAM / TACSTD1 / CD326 HEA125
Synonyms
EPCAM EGP KSA GA733-2 M1S2 M4S1 MIC18 TROP1 Epithelial cell adhesion molecule, Epithelial cell surface antigen
Product review
Please read our product review about Ep-CAM / TACSTD1 / CD326: Antibody panel to Epithelial Cell Adhesion Molecule (EpCAM).
Product Description
Mouse anti Ep-CAM / TACSTD1 / CD326 HEA125, Presentation: Purified. Product is tested for Frozen sections ( C ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | HEA125 |
| Catalog Number | BM2274 |
| Quantity | 50 µg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM2274.pdf |
Datasheet Extract
| Monoclonal Antibody to Ep-CAM//(Human Epithelium Antigen) | |
| Background | The epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34) is widely expressed on cells of epithelial origin and tumors derived therefrom. |
| Immunogen | HT-29 colon carcinoma cell line
|
| Format | State: Lyophilized purified Ig fraction Purification: Protein A chromatography BufferSystem: PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide Label: Restore in 1.0 ml distilled water. |
| Applications | Immunoblotting.
Cell sorting. Cytological material. Immunofluorescence microscopy. Immunohistochemistry on frozen and paraffin-embedded tissue. With paraffin embedded sections, protease pre-treatment is required prior to antibody application. Dilute at least 1:10 with PBS, pH 7.4, for immunohistochemical application. Incubation time: 1 h at RT; extended with paraffin. |
| Specificity | Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein).
HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Reactivities on cultured cell lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Species: Human. |
| Storage | Store original vial at 2-8°C.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | Edwards, P.A.W.: Heterogeneous expression of cell-surface antigens in normal epithelia and their tumors, revealed by monoclonal antibodies. Br. J. Cancer 51, 149-160 (1985)
Hastka, J., Pfiester, P.: Zellmarker zur Differentialdiagnose maligner Mesotheliome. Path. 9, 245-247 (1988) Momburg, F., Moldenhauer, G., Hämmerling, G.J., Möller, P. und Otto, H.F.: Charakterisierung eines epithelspezifischen Zellmembranantigens mit Hilfe des monoklonalen Antikörpers HEA125. Verh. Dtsch. Ges. Path. 69, 627 (1985) Momburg, F., Moldenhauer, G., Hämmerling, G.J., Möller, P.: Immunohistochemical study of the expression of a Mr 34,000 human epitheliumspecific surface glycoprotein in normal and malignant tissues. Cancer Res. 47, 2883-2891 (1987) Joplin, R., Strain, A.J. and Neuberger, J.M.:Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture. J. Pathol. Vol. 162, 255-260 (1990) Racila E, Euhus D, Weiss AJ, Rao C, McConnel J, Terstappen LMWW, and JW Uhr: Detection and characterization of carcinoma cells in the blood. Proc Natl Acad Sci USA 92, 4589-4594 (1998) Balzar M., Winter M.J., deBoer C.J., Litvinov S.V.: The biology of the 17-1A antigen (Ep-CAM). J.Mol.Med. 77, 699-712 (1999) Kommoss F, Oliva E, Young RH, Bittinger F, Kirkpatrick CJ, Schmidt D: Expression of Müllerian inhibiting substance, CD99 and HEA125 in ovarian tumors. Geburtsh Frauenheik 61, 274-279 (2001) Metzgeroth G, Mantz C, Kuhn C, Schultheis B, Hehlmann R, Hastka J: Reliable identification of small cell lung cancer in cytological specimens by immunocytology. Onkologie 30, 311-315 (2007) Metzgeroth G, Kuhn C, Schultheis B, HehlmannR, Hastka J: Diagnostic accurary of cytology and immunocytology in carcinomatous effusions. Cytopathology (Epub 2007) |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'BM2274 Ep-CAM / TACSTD1 / CD326'.
