BM2274X Ep-CAM / TACSTD1 / CD326
see related secondary antibodiessee all 25 Ep-CAM / TACSTD1 / CD326 products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Ep-CAM / TACSTD1 / CD326 HEA125
Synonyms
EPCAM EGP KSA GA733-2 M1S2 M4S1 MIC18 TROP1 FocusOn125 Epithelial cell adhesion molecule, Epithelial cell surface antigen
Product Description
Mouse anti Ep-CAM / TACSTD1 / CD326 HEA125, Presentation: Purified. Product is tested for Frozen sections ( C ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | HEA125 |
| Catalog Number | BM2274X |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM2274X.pdf |
Datasheet Extract
| Monoclonal Antibody to Human CD326 | |
| Alternate names | Ep-CAM |
| Concentration | 0.1 mg/ml |
| Background | This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium. |
| Immunogen | HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line. |
| Format | State: Liquid purified IgG Purification: Affinity chromatography BufferSystem: PBS, pH 7.3 containing 0.09% Sodium Azide, 0.5% Bovine Serum Albumin |
| Applications | Flow Cytometry: Neat.
Immunohistochemistry on frozen sections: 1 : 20. Immunohistochemistry on paraffin sections: 1 : 20; This product requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 minutes. Recommended Positive Control: Human colon and appendix. |
| Specificity | This antibody recognises the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Edwards, P.A.W. (1985) Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies. Br. J. Cancer. 51: 149.
2. Moldenhauer, G. et al. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer. 56: 714-721. 3. Momburg, F. et al. (1987) Immunohistochemical study of the expression of a Mr 34, 000 human epitheliumspecific surface glycoprotein in normal and malignant tissues. Cancer Res. 47: 2883-2891. 4. Simon, B. et al. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc. Natl. Acad. Sci. USA. 87: 2755 ff. 5. Kemmner, W. et al. (1992) Separation of tumour cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. J. Immunol. Methods 147: 197 - 200. 6. Winter , M. J. et al. (2003) The epithelial cell adhesion molecule (Ep - CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163 : 2139 - 2148. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
Click here to see all secondary antibodies for this product.