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BM2364  CD35 antibody

see related secondary antibodies
see all 29 CD35 products
0.2 mg / US$ 675
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD35 J3D3

Synonyms

CR1, C3BR, CD35, C3b/C4b receptor, Complement receptor type 1,

Product Description

Mouse anti CD35 J3D3, Presentation: Azide Free. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN )

Properties

ApplicationsFrozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN )
ReactivityHuman ( Hu )
PresentationAzide Free
HostMouse
IsotypeIgG1
CloneJ3D3
Catalog NumberBM2364
Swiss Prot. No.P17927
Quantity0.2 mg
PriceUS$ 675
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
BM2364.pdf

Datasheet Extract

BackgroundCD35 antigen is composed of 4 allotypes:
C3b Receptor (CR1) present on B Lymphocytes, Monocytes, Myeloid cells, Dendritic cells of Lymphocyte follicules and renal Podocytes.
ImmunogenPurified C3b receptor from human red blood cells.
Swiss Num.: P17927
Format
State: Lyophilized purified Ig fraction.
BufferSystem: PBS with 1 mg/ml BSA as stabilizer and no preservative.
Reconstitution: Restore with 1 ml of distilled water.
ApplicationsMechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with Lymphocytes.
Fluorescence Microscopy or Flow Cytometry: 2 µg/5x10e5 cells/test.
Immunohistochemistry: Working dilution: 1:25 to 1:50. Suitable on frozen sections or cell smears, and on Bouin's fixed, paraffin-embedded tissues.
SpecificityReacts with CD35 also knows as C3b Receptor (CR1).
Species: Human.
StorageStore the reconstituted antibody at 2-8°C for one month (add 0.09% Sodium Azide) or at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Cook J., et al., (1985), Mouse monoclonal antibodies to the human C3b receptor, Molec. Immunol., 22 #5, 531-539.
2. Reid, K.B.M., Complement system protein which interact with C3b or C4d,. Immunology Today, 7, 7.
3. This antibody was studied at the Human Leucocyte Workshop in Oxford (1986).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 315
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 295
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 295
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 305
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 325
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 455
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 455
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 455
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 455
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 455
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 465
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 465
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 465
   

Click here to see all secondary antibodies for 'BM2364 CD35'.