BM2368 CD19 / Leu-12 antibody
see related secondary antibodiessee all 86 CD19 / Leu-12 products
0.2 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD19 / Leu-12 J4.119
Synonyms
CD19, Differentiation antigen CD19, B-lymphocyte surface antigen B4, Leu-12, B-lymphocyte antigen CD19,
Product Description
Mouse anti CD19 / Leu-12 J4.119, Presentation: Azide Free. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN ) |
| Reactivity | Human ( Hu ) |
| Presentation | Azide Free |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | J4.119 |
| Catalog Number | BM2368 |
| Swiss Prot. No. | P15391 |
| Quantity | 0.2 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM2368.pdf |
Datasheet Extract
| Alternate names | Monoclonal Antibody to CD19, gp95 |
| Concentration | 0.2 mg/ml (prior to lyophilization) |
| Background | CD19 antigen is a single chain, type I integral membrane glycoprotein with a molecular weight of 95 kDa. |
| Immunogen | SKLY 18 lymphoma cells. Swiss Num.: P15391 |
| Format | State: Lyophilized purified Ig fraction. Purification: Protein A Sepharose chromatography. BufferSystem: PBS with 1 mg/ml BSA and no preservatives. Reconstitution: Restore with 1 ml distilled water. |
| Applications | Study of CD19 expressing Lymphocytes.
Flow Cytometry: 2 µg/test (5x10e5 cell/test) or 100 µl blood sample. Immunohistochemistry: Working dilution 1:25-1:50. This antibody is suitable on cryostat sections. |
| Specificity | Expression of the CD19 molecule is found on all B Lymphocytes, including pro-B cells, but is lost during maturation to plasma cells.
The CD19 antigen is also expressed on the membrane of follicular dendritic cells and on most stabilized B cell lines. CD19 expression is not observed in normal T Lymphocytes, NK cells, Monocytes and Granulocytes. CD19 appears important in regulating B-cell activation and proliferation. In vitro studies show that the CD19 antibody has an inhibitory effect on the activation and proliferation of B Lymphocytes. |
| Storage | Store the reconstituted antibody at 2-8°C for one month (add 0.09% Sodium Azide) or at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Nadler, L.M., (1983), Journal of Immunology, 131, 244-247.
2. Melson, H., (1984), Scand. J. Haematol., 33, 27-34. 3. Campana, D. et al., (1986), Blood, 68, 1264-1271. 4. Zola, H., (1987), Immunology Today, 10, 308-315. 5. Freedman, A.S., et al., (1987), Seminars in Oncology, 14, 193-212. 6. Pezzuto, A. et al., (1987), in Leucocyte Typing III, White Cell Differentiation Antigens, 358-360. 7.This antibody was studied at the human leucocyte workshop in Oxford (1986). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM2368 CD19 / Leu-12'.