BM3359 CD227 / Mucin-1 antibody
see related secondary antibodiessee all 70 CD227 / Mucin-1 products
1 mg / US$ 645
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD227 / Mucin-1 695
Synonyms
CA15-3, MUC-1, Polymorphic epithelial mucin, PEM, PEMT, Episialin, Tumor-associated mucin, Carcinoma-associated mucin, EMA, H23AG, PUM, Tumor-associated epithelial membrane antigen, nut-reactive urinary mucin, Breast carcinoma-associated antigen DF3
Product Description
Mouse anti CD227 / Mucin-1 695, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 695 |
| Catalog Number | BM3359 |
| Swiss Prot. No. | P15941 |
| Quantity | 1 mg |
| Price | US$ 645 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM3359.pdf |
Datasheet Extract
| Alternate names | Mucin 1, MUC1 |
| Concentration | 2.5 mg/ml (OD280 nm, E0.1% = 1.35) |
| Background | The MUC-1 mucin (Cancer Antigen 15-3) is secreted from tumor cells.
The apo-protein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). |
| Immunogen | Human breast cancer cell line ZR 75-1 Swiss Num.: P15941 |
| Format | State: Liquid purified IgG fraction (>98% pure). Purification: Protein A chromatography. BufferSystem: 0.15 M NaCl without preservatives. |
| Applications | Suitable for use in ELISA (Capture or detection antibody in serological assays for the research of antigen MUC-1. Forms a suitable pair with clone 552 (Cat. No. BM3358)
Immunohistochemistry (on frozen or paraffin sections. Western blot. |
| Specificity | Clone 695 recognizes a sialylated carbohydrate epitope of the MUC-1 glycoprotein. It is sensitive to periodate and sialidase treatment, but it does not react with glycolipid extract from the ZR 75-1 cell line. Species: Human. |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Hilkens J., Buijs F., Hilgers J., Hageman P., Calafat J., Sonnenberg A., and van der Valk J. Int J. Cancer 34, 197, 1984.
2. Baeckstrom D., Nilsson O., Price MR, Lindholm L., and Hansson GC. Discrimination of MUC1 mucins from other Sialyl-Lea-carrying glyco-proteins by colon carcinoma cells using a novel monoclonal antibody. Cancer Res., 53, 755, 1993. 3. Nilsson O., Karlsson B., Lindholm L., Petersson A. Development of assays for determination of MUC-1 breast cancer antigen, 7th Symposium on Tumors Markers, Hamburg 1993, R Klapdor (ed) Current Tumor Diagnostics: Applications, Clinical Relevance, Research, Trends. W. Zuckerwerdt Verlag Munchen, 1994. 4. Nilsson O., Karlsson B., Lindholm L., Petersson A. Novel Mab for determination of breast cancer associated MUC-1 mucin antigen. XXII International Society for Oncodevelopment Biology and Medicine, Groningen 1994. 5. Perey L. et al., Tumor Selective Reactivity of a Monoclonal Antibody Prepared against a Recombinant Peptide Derived from the DF3 Human Breast Carcinoma-associated Antigen.Cancer Reasearch 52, 2563-2568. May 1, 1992. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM3359 CD227 / Mucin-1'.
