BM4000 CD68 / Macrosialin antibody
see related secondary antibodiessee all 46 CD68 / Macrosialin products
0.25 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD68 / Macrosialin ED1
Synonyms
CD68, Gp110, Macrosialin,
Product Description
Mouse anti CD68 / Macrosialin ED1, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | ED1 |
| Catalog Number | BM4000 |
| Swiss Prot. No. | P34810 |
| Quantity | 0.25 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4000.pdf |
Datasheet Extract
| Alternate names | MACROSIALIN |
| Concentration | 0.5 mg/ml IgG |
| Immunogen | Rat spleen cells
Swiss Num.: P34810 |
| Format | State: Liquid IgG fraction Purification: Protein A affinity chromatography BufferSystem: Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide as preservative |
| Applications | Flow cytometry (use 10 µl of 1/50 - 1/100 diluted antibody to label 10e6 cells in 100 µl; results may be enhanced using membrane permeabilisation).
Immunohistology on frozen sections. Immunohistology on paraffin sections (1/100, requires protein digestion pretreatment of paraffin sections with pronase or trypsin). Western blotting. Immunoprecipitation. RIA. |
| Specificity | Antibody BM4000 recognises a single chain glycoprotein of 110 kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Species: Rat. |
| Add. Information | Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68. |
| Storage | Store the antibody at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life one year from despatch. |
| References | 1. Damoiseaux, J.G.M.C. et al. (1994). Rat macrophage lysosomal membrane antigen recognised by monoclonal antibody ED1. Immunol. 83: 140-147.
2. Dijkstra, C.D. et al. (1985). The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2 and ED3. Immunol 54: 589. 3. Bauer, J. et al. (1994). Phagocytic activity of macrophages and microglial cells during the course of Acute and Chronic Relapsing Experimental Autoimmune Encephalomyelitis. J. Neurosci. Res. 38: 365-375. 4. Honing, W. et al. (2003). The rat macrophage ED1 antigen is identical to CD68 and mediates phagocytosis of oxidized low-density lipoproteins. (submitted). 5. Bao, F. et al. (2004). Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J, Neuro-chem. 88:1335-1344. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4000 CD68 / Macrosialin'.
