BM4001 CD163 antibody
see related secondary antibodiessee all 19 CD163 products
0.25 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD163 ED2
Synonyms
CD163, M130, Hemoglobin scavenger receptor, , Scavenger receptor cysteine-rich type 1 protein M130
Product Description
Mouse anti CD163 ED2, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | ED2 |
| Catalog Number | BM4001 |
| Swiss Prot. No. | Q2VLH6 |
| Quantity | 0.25 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4001.pdf |
Datasheet Extract
| Alternate names | ED2 |
| Concentration | 0.5 mg/ml |
| Background | CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells. |
| Immunogen | Rat Spleen cell homogenate Swiss Num.: Q2VLH6 |
| Format | State: Liquid, purified IgG Purification: Protein A affinity chromatography BufferSystem: PBS, pH 7.4, with 0.09% sodium azide as preservative |
| Applications | Immunohistology: 1:50-1:100 for frozen sections.
This product requires antigen retrieval using pronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to ref. Whiteland et al (5.) Histology positive control tissue: liver. Flow cytometry (use 10 µl of 1:10 - 1:100 diluted antibody to label 10e6 cells in 100 µl). Immunoprecipitation. Western blot. |
| Specificity | BM4001 recognises the CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. Species: Rat. Does not react with Human. |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Dijkstra, C.D., Dopp, E.A., Joling, P. and Kraal, G. (1985). Immunology 54: 589.
2. Beelen, R.H.J., Eestermans, I.L., Dopp, E.A. and Dijkstra, C.D. (1987). Transpl. Proc. 19: 3166. 3. Barbe, E., Damoiseaux, J.G.M.C., Dopp, E.A. and Dijkstra, C.D. (1990) Immunobiol. 182-188. 4. Dijkstra, C.D. & Damoiseaux, J.G.M.C. (1993). Progress in Histochemistry and Immunohistochemistry 27: 1-65. 5. Whiteland, J.L. et al. (1995). Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320. 6. Polfliet, M.M.J. et al (2002). Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators. PhD Thesis. Vrije University, Amsterdam. 7. Deng, X. et al. (2005). Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats. Am. J. Pathol. 166: 93-106. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4001 CD163'.
