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Staining of rat periton

 

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BM4001R  CD163 antibody

see related secondary antibodies
see all 19 CD163 products
100 Tests / US$ 575
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD163 ED2

Synonyms

CD163, M130, Hemoglobin scavenger receptor, , Scavenger receptor cysteine-rich type 1 protein M130

Product Description

Mouse anti CD163 ED2. Product is tested for Flow cytometry ( F )

Properties

ApplicationsFlow cytometry ( F )
ReactivityRat ( Rt )
PresentationPE
HostMouse
IsotypeIgG1
CloneED2
Catalog NumberBM4001R
Swiss Prot. No.Q2VLH6
Quantity100 Tests
PriceUS$ 575
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
BM4001R.pdf

Datasheet Extract

Alternate namesED2
BackgroundCD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
ImmunogenRat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Swiss Num.: Q2VLH6
Format
State: Lyophilized purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS. pH 7.4 containing 0.09% sodium azide and 1% BSA
Label: R. Phycoerythrin (RPE)
Reconstitution: Restore with 1 ml distilled water.
ApplicationsFlow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
SpecificityThsi antibody recognises the CD163 cell surface glycoprotein, a 175kD molecule also known as ED2.

Species: Rat.
StoragePrior to and following reconstitution store the antibody at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
References1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 54:589-599.
2. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation. Transplant. Proc. 3: 3166-3170.
3. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2. Immunobiol. 182: 88-99.
4. Dijkstra, C.D. and Damoiseaux, J.G.M.C. (1993) Macrophage heterogeneity established by
immunocytochemistry. Prog. Histochem. Cytochem. 27: 1-65.
5. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators. PhD Thesis. Vrije University, Amsterdam.
PicturesStaining of rat periton

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 315
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 295
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 295
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 305
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 325
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 455
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 455
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 455
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 455
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 455
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 465
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 465
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 465
   

Click here to see all secondary antibodies for 'BM4001R CD163'.