BM4002 CD169 / Sialoadhesin antibody
see related secondary antibodiessee all 6 CD169 / Sialoadhesin products
0.25 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD169 / Sialoadhesin ED3
Synonyms
SIGLEC1, SN, ED3, CD169, Siglec-1, Sialic acid-binding Ig-like lectin 1, Sialoadhesin, ,
Product review
Please read our product review about CD169 / Sialoadhesin: Antibodies to Rat Macrophages.
Product Description
Mouse anti CD169 / Sialoadhesin ED3, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | ED3 |
| Catalog Number | BM4002 |
| Swiss Prot. No. | Q62230 |
| Quantity | 0.25 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4002.pdf |
Datasheet Extract
| Alternate names | Marginal Zone Metallophils; ED3 |
| Concentration | 0.5 mg/ml |
| Background | CD169 is a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid. |
| Immunogen | Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line. Swiss Num.: Q62230 |
| Format | State: Liquid purified IgG Purification: Affinity chromatography on Protein A BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide |
| Applications | Immunoprecipitation.
Immunohistochemistry on frozen sections: 1/500. Immunohistochemistry on paraffin sections: 1/250. |
| Description | ED 3 is a characteristic marker for tissue fixed macrophage subpopulations, particularly of lymphoid organs (e.g. marginal metallophilic macrophages). No other cell types, including monocytes and granulocytes, are positive. Macrophages in autoimmune diseased but not in healthy tissuess express the ED3 antigen. The recognised antigen appears to be a receptor for sialylated glycoconjugates. |
| Specificity | This antibody recognises the CD169 cell surface antigen. Species: Rat. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phageocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2, ED3. Immunology 54: 589-594.
2. Van Rees, E.P. et al. (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study. Cell Tissue. Res. 242: 391-398. 3. Van den Berg, T.K. et al. (1992) Sialoadhesin on macrophages, its identification as lymphocyte adhesion molecule. J. Exp. Med. 176: 647-655. 4. Honing, W. et al. (2003) Characterization of rat sialoadhesin. (submitted) |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4002 CD169 / Sialoadhesin'.
