BM4003 Macrophages (pan tissue fixed) antibody
see related secondary antibodiessee all 58 Macrophages products
0.1 mg / US$ 525
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages (pan tissue fixed) Ki-M2R
Synonyms
Rat Macrophage Marker, ,
Product review
Please read our product review about Macrophages (pan tissue fixed): Antibodies to Rat Macrophages.
Product Description
Mouse anti Macrophages (pan tissue fixed) Ki-M2R, Presentation: Purified. Product is tested for Frozen sections ( C ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | Ki-M2R |
| Catalog Number | BM4003 |
| Quantity | 0.1 mg |
| Price | US$ 525 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4003.pdf |
Datasheet Extract
| Immunogen | Rat peritoneal macrophages |
| Format | State: Lyophilized Ig fraction Purification: Affinity chromatoghraphy BufferSystem: Phosphate buffered saline pH 7.2 (PBS), 5 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.09 % sodium azide as a preservative Reconstitution: Restore in 0.5 ml distilled water to a concentration of 0.2 mg/ml. |
| Applications | Immunohistochemistry on frozen sections (1:400).
Immunohistochemistry on paraffin sections (1:40; Proteinase K pretreatment for antigen retrieval is recommended). Suggested positive control: Rat spleen. |
| Specificity | BM4003 recognizes typical tissue macrophages and specifically discriminates between monocytes (Ki-M2R-) and macrophages(Ki-M2R+). It stains all resident macrophages including those of bone marrow, lymphatic sinuses, lymphoid follicles, splenic red pulp, and Kupffer cells in the liver. Langerhans cells, interdigitating reticulum cells, and dendritic reticulum cells of lymphoid follicles are not stained.
Ki-M2R recognises a surface antigen of 45kD molecular weight as a single band under reducing conditions. The epitope has not been further characterized. Antigen distribution: Isolated cells: In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoetic cells and megacaryocytes are negative. Immature accessory cells such as dendritic or interdigitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material. Tissue sections: Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kupffer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50 % of alveolar macrophages) and connective tissue (histiocytes) are positively stained. Plasma cells (excluding stimulated monocytes) as well as sinus lining cells are negative. Species: Rat. |
| Storage | Store lyophilized at 2 - 8 °C and reconstituted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. |
| References | WACKER, H.H. et al.: Ki-M2R a new specific monoclonal antibody, discrimates tissue macrophages from reticulum cells and moncytes in vivo and in vitro. J.Leuk.Biol.: 38, 509 - 520 (1985).
WACKER, H.H. et al.: Kinetics of Kuppfer cells as shown by parabiosis and combined autoradiographic/immunohistochemical analysis. Virch.Arch. B. (Cell Pathol.): 51, 71 - 78 (1986). WACKER, H.H. et al.: Selective recognition of Rat Follicular Dendritic Cells (Dendritic Reticulum Cells) by a New Monoclonal Antibody KI-M4R In Vitro and In Vivo. J. Leuk. Biol. 41, 70-77 (1987). |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1 h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 12. Wash in PBS 13. Counterstain with Mayer's hemalum |
| Pictures | Frozen rat splee, stained with BM4003. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4003 Macrophages (pan tissue fixed)'.
