BM4004 Macrophages (pan) antibody
see related secondary antibodiessee all 58 Macrophages products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages (pan) RM1
Synonyms
Rat Macrophage Marker, ,
Product review
Please read our product review about Macrophages (pan): Antibodies to Rat Macrophages.
Product Description
Mouse anti Macrophages (pan) RM1, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | RM1 |
| Catalog Number | BM4004 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4004.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Immunogen | Rat Peritoneal Macrophages. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS pH 7.2, 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Immunohistochemistry on:
Frozen sections: 2 µg/ml, 1/100. Paraffin sections: 20 µg/ml, 1/10, microwave pretreatment for antigen retrieval is recommended. Has been described to work in FACS. Suggested positive control: Rat spleen. |
| Specificity | This antibody detects monocytes, subpopulation of macrophages, interdigitating cells.
The antigen is localized on the surface of cells. It is stable to 2% periodate-lysine-paraformaldehyde fixation. The epitope has not been further characterized. Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % of peritoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells. Species: Rat. |
| Storage | Store lyophilized product at 2-8 °C.
Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | TAKEYA, M.et al.: Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study. J. Histochem Cytochem: 37: 635-641 (1989).
KATO, T. et al.: Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analysis with immunohistochemistry, immunoelectron microscopy and 3H thymidine autoradiography. Lab Invest: 62: 635-645 (1990). IZUMI, S. et al.: Ontogenic Development of synovial A cells in fetal and neonatal rat knee joints. Cell Tiss Res : 262: 1-8 (1990). KASPER, M. et al.: Heterogenous Dolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem 95: 1-11 (1993). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4004 Macrophages (pan)'.
