BM4013 B-Cells (pan) antibody
see related secondary antibodiessee all 41 B-Cells products
50 µg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti B-Cells (pan) Ki-B1R
Synonyms
B Cells
Product Description
Mouse anti B-Cells (pan) Ki-B1R, Presentation: Purified. Product is tested for Flow cytometry ( F ), Frozen sections ( C ), Paraffin Sections ( P )
Properties
| Applications | Flow cytometry ( F ), Frozen sections ( C ), Paraffin Sections ( P ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | Ki-B1R |
| Catalog Number | BM4013 |
| Quantity | 50 µg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4013.pdf |
Datasheet Extract
| Monoclonal Antibody to Rat B-Lymphocytes | |
| Concentration | 0.1 mg/ml (after reconstitution) |
| Immunogen | Rat lymphocytes
|
| Format | State: Lyohilized affinity purified IgG fraction BufferSystem: PBS, pH 7.2, containing 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative Reconstitution: Restore by adding 0.5 ml distilled water. |
| Applications | Immunohistochemistry: Frozen sections: 1 µg/ml (1:100), Paraffin sections: 10 µg/ml (1:10).
No antigen retrieval required, microwave treatment may enhance the reactivity. Suggested positive control: Rat spleen. Has been described to work in FACS. |
| Description | Ki-B1R is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and cryostat sections of rat tissues. Immungen: rat lymphocytes. Format: This antibody is supplied affinity purified and lyophilized. Reconstitute by adding 0.5 ml distilled water. This stock solution contains 0.1 mg/ml IgG, PBS pH 7.2, 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative. |
| Specificity | Monoclonal rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections.
Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. The antibody is specific for rat mature B-lymphocytes and plasma cells. Other species not tested. Staining of isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% +/- 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue staining: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. |
| Storage | Original vial: 1 year from despatch at 2-8°C.
Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated freezing and thawing. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4013 B-Cells (pan)'.
