BM4021 CD163 antibody
see related secondary antibodiessee all 19 CD163 products
0.2 mg / US$ 505
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD163 RM3/1
Synonyms
CD163, M130, Hemoglobin scavenger receptor, , Scavenger receptor cysteine-rich type 1 protein M130
Product Description
Mouse anti CD163 RM3/1, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Monkey ( Mky ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | RM3/1 |
| Catalog Number | BM4021 |
| Swiss Prot. No. | Q86VB7 |
| Quantity | 0.2 mg |
| Price | US$ 505 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4021.pdf |
Datasheet Extract
| Alternate names | Intermediate stage inflammatory macrophages |
| Background | CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions. |
| Immunogen | Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture Swiss Num.: Q86VB7 |
| Format | State: Lyophilized Ig fraction Purification: Affinity chromatography BufferSystem: Phosphate buffered saline pH 7.2 (PBS), 10 mg/ml BSA as a stabilizer and 0.01 % thimerosal as a preservative Reconstitution: Restore with 0.5 ml distilled water to a concentration of 0.4 mg/ml. |
| Applications | Immunohistochemistry on frozen sections (1:100). Does not work in routinely processed paraffin sections.
Has been described to work in FACS and Western blots. Suggested positive control: Human placenta. |
| Specificity | This antibody is specific for CD163.
Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues. Species: Human, Monkey. Not Pig, Rabbit and Guinea Pig. |
| Storage | Store the lyophilized product at 2 - 8 °C. Following reconstitution store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. |
| References | Zwadlo, G. et al.: A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp.Cell Biol 55: 295-304 (1987)
Zwadlo-Klarwasser, G. et al.: Glucocorticoid-induced appearence of the macrophage subtype RM 3/1 in peripheral blood of man. Int Arch Allergy Appl. Immunol 91: 175-180 (1990) Högger, P. et al.: J. Immunology 161: 1883-1890 (1998). |
| Pictures | Frozen human placenta, stained with BM4021 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4021 CD163'.
