BM4032B CD11b / ITGAM antibody
see related secondary antibodiessee all 118 CD11b / ITGAM products
0.75 mg / US$ 415
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD11b / ITGAM X-5
Synonyms
ITGAM, CD11B, CR3A, Integrin alpha-M, CR-3 alpha chain, , Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11 antigen-like family member B
Product Description
Mouse anti CD11b / ITGAM X-5, Presentation: Biotin. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Presentation | Biotin |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | X-5 |
| Catalog Number | BM4032B |
| Swiss Prot. No. | P11215 |
| Quantity | 0.75 mg |
| Price | US$ 415 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4032B.pdf |
Datasheet Extract
| Alternate names | Complement C3bi Receptor, MAC-1 |
| Concentration | 1.5 mg/ml |
| Background | Antibody X-5 recognizes a non-covalently bound alpha heterodimeric glycoprotein. It belongs to the Ã?-2 integrin supergene family of adhesion. The antigen is a receptor for C3bi, fibrinogen, coagulation factor X. Together with CD18, CD11b forms the MAC-1 molecular complex involved in the adhesion to endothelial cells, and the subsequent extravasation of neutrophils and monocytes. |
| Immunogen | Human peripheral blood monocytes Swiss Num.: P11215 Remarks: PBS, pH 7.2 containing 5 mg/ml bovine serum albumin (BSA) as a stabilizer and Kathon as a preservative. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS, pH 7.2 with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer. Label: Biotin. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections: 7.5 - 30 µg/ml (1/50-1/200).
Suggested positive control: Human tonsil Antigen Distribution on Isolated cells and Tissue sections: Weakly positive on granulocytes. Positive on peritoneal macrophages. Negative on lymphocytes. Cell Lines: HL-60: positive on approx. 50% following 1.25% DMSO treatment increased to 100%. In lymph nodes X-5 is positive on monocytes, macrophages and single cells both intrafollicularly and in surrounding blood vessels. Positive staining has been observed with the basal membrane of blood vessels and on sinus walls. Marginal zone macrophages, medulla and red pulp stain positive in the spleen as do cells in the T-cell region. In the thymus, medulla and trabecular macrophages are positive. |
| Specificity | This antibody reacts with granulocytes, monocytes, NK- cells.
X-5 recognizes an antigen of 155/95kD determined by Immunoprecipitation. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Breard, J. et al.: J. Immunol. 124, 1943, (1980).
2. Ault, K.A. & SPRINGER, T.A.; J. Immunol. 126, 359, (1981). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4032B CD11b / ITGAM'.