BM4034 CD14 antibody
see related secondary antibodiessee all 129 CD14 products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD14 X-8
Synonyms
CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14 antigen, Monocyte differentiation antigen CD14,
Product Description
Mouse anti CD14 X-8, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | X-8 |
| Catalog Number | BM4034 |
| Swiss Prot. No. | P08571 |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4034.pdf |
Datasheet Extract
| Alternate names | Endotoxin (LPS) Receptor |
| Concentration | 0.2 mg/ml (stock solution) |
| Background | CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14. |
| Immunogen | Human monocytes. Swiss Num.: P08571 |
| Format | State: Lyophilised Purification: Affinity purified IgG BufferSystem: PBS pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative. Reconstitution: Reconstitute by adding 0.5 ml distilled water = stock solution. |
| Applications | Tested for immunohistochemistry (IHC) on frozen sections: use at 2-4 μg/ml (1:50 - 1:100). Does not react on routinely processed Paraffin sections. Suggested positive control: Human placenta.
Has been described to work in FACS (post fixation is recommended). |
| Specificity | Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14.
BM4034 is specific for human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized. Species: Human. |
| Add. Information | Antigen distribution on isolated cells: Monocytes, granulocytes, myeloid leukaemia and myeloid cell lines.
Antigen distribution on tissue sections: Positive on histiocytes, Langerhans cells, follicular dendritic cells and high endothelial venules. |
| Storage | Store the original vial at 2-8°C, stock solution or aliquots thereof at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | SHAW, S. et al. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press (1994) Ed. Schlossmann,S. et al. Abstract M100 (X-8 Workshop Code = MA82)
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4034 CD14'.