BM4035 Macrophages antibody
see related secondary antibodiessee all 58 Macrophages products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages X-4
Synonyms
Macrophage marker,
Product Description
Mouse anti Macrophages X-4, Presentation: Purified. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | X-4 |
| Catalog Number | BM4035 |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4035.pdf |
Datasheet Extract
| Alternate names | Possibly Recognizing A Scavenger Receptor |
| Concentration | 0.2 mg/ml |
| Immunogen | Cultured human monocytes. |
| Format | State: Lyophilized purified Ig fraction Purification: Affinity chromatography BufferSystem: PBS, pH 7.2 containing 0.01% thiomerosal as preservative and 10 mg/ml BSA as stabilizer. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections: 2 µg/ml (1/100).
Suggested positive control: Human placenta. Antigen distribution on Isolated cells and Tissue sections: Positive on peritoneal macrophages. Positive on cultivated macrophages under serum supplementation. Negative under serum-free conditions. Negative on freshly isolated monocytes. Positive in AML but not on blast cells. Negative on fMLP activated neutrophils. U937 show increased antigen expression after TNFα and GM-CSF treatment. X-4 is positive on macrophages, Kupffer cells, mesangial cells and Hoffbauer cells. It is positive on intrafollicular and paracortical dendritic and spindel macrophages and with macrophages and foamy cells in Xathoma disseminatum but negative in sarcoid granuloma and histiocytosis X. |
| Specificity | X-4, together with X-14, and PM-2K, forms a particular group of macrophage specific antibodies which were classified at the V Leukocyte Typing Workshop held in Boston in 1993. The X-4 antigen may function as a scavenger receptor.
Detects human macrophages (main cellular reactivity). Cross reactivity: positive with rabbit, cat, dog, goat, pig, horse, monkey, weak on guinea pig, negative with bovine and rat. The antigen is a protein of 100kD as determined by immunoprecipitation. It is found on the cytoplasmic membranes of macrophages. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Xu, H. et al.: Identification of Differentiation Antigens on Human Monocytes and Macrophages with Newly Produced Monoclonal Antibodies. Exp. Cell. Biol. 57, 122 (1989).
2. Shaw, S. e al.: Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press (1994) Ed. Schlossmann,S. et al. Abstracts M017, M020, M035, M061, M081, M085, M105, M108, M132, M133, M138, M145, Suppl. (X-4 Workshop Codes = M49, BP262) 3. Zeng, L. et al.: Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species. J Histochem Cytochem 44: 845-853. (1996) |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum. |
| Pictures | Table 1. Comparison of Staining Patterns of X-4 (BM4035), X-14 (BM4036) and PM-2K (BM4037). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4035 Macrophages'.
