BM4036 Macrophages antibody
see related secondary antibodiessee all 59 Macrophages products
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages X-14
Synonyms
Macrophage marker,
Product Description
Mouse anti Macrophages X-14, Presentation: Purified. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | X-14 |
| Catalog Number | BM4036 |
| Quantity | 0.1 mg |
| Price | US$ 425 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4036.pdf |
Datasheet Extract
| Alternate names | Possibly Recognizing A Scavenger Receptor |
| Concentration | 0.2 mg/ml |
| Background | Monoclonal antibody X-14, together with X-4, and PM-2K, forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop
held in Boston in 1993. The X-14 antigen was initially suspected to function as a scavenger receptor. |
| Immunogen | Cultured human monocytes. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS, pH 7.2 with 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections: 2 µg/ml (1/100).
Suggested positive control: Human tonsil. Antigen distribution on Isolated cells and Tissue sections: Positive on peritoneal macrophages and on cultivated macrophages with serum supplementation. Positive in AML but not on blast cells. Negative under serum-free conditions, on freshly isolated monocytes and on fMLP activated neutrophils. U937 cells show increased antigen expression after TNFα and GM-CSF treatment. X-14 is positive on macrophages, Kupffer cells, mesangial cells and Hoffbauer cells. It is positive on intrafollicular and paracortical dendritic and spindel macrophages and with macrophages and foamy cells in Xathoma disseminatum but negative in sarcoid granuloma and histiocytosis X. |
| Description | X-14, together with X-4, and PM-2K forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop held in Boston in 1993. The X-14 antigen was initially suspected to function as a scavenger receptor. However, experiments with CHO cells expressing MSR AI and MSR AII showed that they were not recognized by this antibody. Format: This purified antibody was lypophilised from PBS buffer pH 7.4 with 0.01% thimerosal as preservative and 10 mg/ml BSA as stabilizer. Reconstitute in 0.5ml distilled water (= 0.2 mg/ml stock solution). |
| Specificity | Human macrophages; also positive on monkey, weak on rabbit, goat, bovine and horse. Negative on rat, guinea pig, cat, and dog. The antigen is found on the cytoplasmic membranes of macrophages.
Experiments with CHO cells expressing Macrophage Scavenger Receptor (MSR) AI or AII showed that they were not recognized by this antibody. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. XU,H., STEINHAUSEN,F., PETERS,J.H.: Identification of Differentiation Antigens on Human Monocytes and Macrophages with Newly Produced Monoclonal Antibodies. Exp. Cell. Biol. 57, 122 (1989).
2. SHAW,S. et al. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press (1994) Ed. Schlossmann,S. et al. Abstracts M017, M030, M060, M081, M105, M132, M138, M145 (X-14 Workshop Codes = M51, BP264) 3. Zeng, L., M. Takeya, X. Ling, A. Nagasaki, K. Takahashi.: Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species. J Histochem Cytochem 44: 845-853. (1996) |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum. |
| Pictures | Table 1. Comparison of Staining Patterns of X-4 (BM4035), X-14 (BM4036) and PM-2K (BM4037) on different cell types. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4036 Macrophages'.
