BM4041B CD163 antibody
see related secondary antibodiessee all 19 CD163 products
0.1 mg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD163 5C6-FAT
Synonyms
CD163, M130, Hemoglobin scavenger receptor, , Scavenger receptor cysteine-rich type 1 protein M130
Product Description
Mouse anti CD163 5C6-FAT, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Biotin |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 5C6-FAT |
| Catalog Number | BM4041B |
| Swiss Prot. No. | Q86VB7 |
| Quantity | 0.1 mg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4041B.pdf |
Datasheet Extract
| Alternate names | Haemoglobin-Haptoglobin Receptor, Scavenger Receptor |
| Concentration | 0.2 mg/ml |
| Background | CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells.
CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions. |
| Immunogen | Human CD163
The epitope has not been further characterized. Swiss Num.: Q86VB7 |
| Format | State: Lyophilized purified IgG fraction Purification: Affinity chromatography BufferSystem: PBS, pH 7.2, containing 5 mg/ml BSA and 0.09% sodium azide as preservative Label: Biotin Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Tested for immunohistochemistry (IHC) and ELISA; has been described to work in FACS and Western blots.
Approximate working dilution for IHC: Frozen sections: 0.5 μg/ml (1:400) Paraffin sections: 1 μg/ml; Proteinase K pretreatment for antigen retrieval recommended. Suggested positive control: Human placenta. |
| Specificity | Clone 5C6 FAT recognizes CD163, a membrane glycoprotein on human monocytes and macrophages which is expressed in intermediate and late inflammatory stages.
Antigen distribution on isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Tissue sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, andcortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody BM4022 (clone 25F9) which is not the case in other tissues. Species: Human. |
| Storage | Store original vial at 2-8°C.
After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | Högger, P. et al.: J. Immunology 161: 1883-1890 (1998) |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4041B CD163'.
