BM4049 CD49B / ITGA2 antibody
see related secondary antibodiessee all 52 CD49B / ITGA2 products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD49B / ITGA2 A.1.43
Synonyms
ITGA2, Platelet membrane glycoprotein Ia, GPIa, Collagen receptor, VLA-2 alpha chain, CD49 antigen-like family member B, CD49b, Integrin alpha-2,
Product Description
Mouse anti CD49B / ITGA2 A.1.43, Presentation: Purified. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | A.1.43 |
| Catalog Number | BM4049 |
| Swiss Prot. No. | P17301 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4049.pdf |
Datasheet Extract
| Alternate names | Integrin α2 Chain, VLA-2α Chain, Platelet gp1a |
| Concentration | 0.2 mg/ml |
| Immunogen | Swiss Num.: P17301 |
| Format | State: Lyophilized purified IgG fraction. Purification: Affinity chromatography. BufferSystem: PBS buffer pH 7.2 with 0.01% Thimerosal as preservative and 1% BSA as stabilizer. Reconstitution: Restore with 0.6 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections (0.25-2 µg/ml, 1/100-1/800) and FACS.
Suggested positive control: Human skin. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19, SK-Mel-29, SK-Mel-113, and negative on normal monocytes. |
| Specificity | The antibody A.1.43 detects CD49b and is a useful tumor progression marker, particularly also in combination with clone A.1.43 detects CD49b and is a useful tumour progression marker, particularly also in combination with clone A10-33/1 (anti gpIIb/IIIa).
CD49b, integrin α2, is a transmembrane glycoprotein that is non-covalently associated with the integrin β1 chain to form the α2β1 (very late antigen (VLA) 2) complex. The α2β1 complex was originally reported on long-term activated T cells and was later shown to be identical to the gpIa/IIa complex on platelets. The α2β1 complex is a pivotal receptor for collagen, and the adhesion of platelets to collagen is known to be important for the initial step of platelet aggregation. The antibody stains an epitope on the cell surface. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19, SK-Mel-29, SK-Mel-113, and negative on normal monocytes. Tissue sections: Acetone fixed tissue sections of melanoma larger than 1.5 mm show positive staining whereas the majority of benign melanocytic nevi (>95%) are negative. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Klein, C.E., et al.: Identification of a melanoma progression antigen as integrin VLA-2. J. Invest. Dermatol. 96: 281-84 (1991)
2. Broecker, E.B. et al.: Phenotypic dynamics of tumor progression in human malignant melanoma. Int. J. Cancer 36: 29-35 (1985) 3. Holzmann, B. et al.: Tumor progression in human malignant melanoma: five stages defined by their antigenic phenotypes. Int. J. Cancer 36: 466-71 (1987) 4. Yamamura, K. & Mishima, Y.: Antigen dynamics in melanocytic proteoglycan expression oncogenesis: anti-ganglioside and anti-ras p21 antibodies as markers of tumor progression. J. Invest. Dermatol. 94: 174-82 (1986) 5. Danen E.H.J. et al:: Emergence of alpha-5 beta-1 fibronectin- and alpha-v beta-3 vitronectin receptor expression in melanocytic tumor progression. Histopathology 24: 249-56 (1994) |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum For further information and details see technical information |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4049 CD49B / ITGA2'.