BM4077 Mucin-like carcinoma antigen antibody
see related secondary antibodies
50 µg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Mucin-like carcinoma antigen b-12
Product Description
Mouse anti Mucin-like carcinoma antigen b-12, Presentation: Ascites. Product is tested for Frozen sections ( C ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | b-12 |
| Catalog Number | BM4077 |
| Quantity | 50 µg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4077.pdf |
Datasheet Extract
| Alternate names | Mucin-like Carcinoma Antigen, MCA |
| Concentration | 0.1 mg/ml |
| Immunogen | Breast carcinoma cell lines. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography BufferSystem: PBS, buffer pH 7.2 with 0.05% sodium azide as preservative and 2 mg/ml BSA as stabilizer Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on:
Frozen sections: 0.5 µg/ml (1/200). Paraffin sections: 4 µg/ml (1/25); Proteinase K pretreatment for antigen retrieval recommended. Suggested positive control: Human uterus. Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is only located at the MCA producing sites such as glandular cell surfaces or glandular tubuli. In MCA producing tumors, where cells become disorganized, the b-12 antigen is secreted into stromal tissue and blood vessels. (See Table 1.) |
| Specificity | b-12 is useful for identifying mucin-like carcinoma antigen (MCA) produced by various tumors and certain healthy glandular cells. In combination with other BMA markers for inflammation staging or investigating neo-vascularization processes b-12 is a valuable tool for studying tumor growth or regression. MCA is a 350 kDa glycoprotein with the typical biochemical characteristics of mucin-like glycoproteins (sialomucins) which protect surfaces. Antibody b-12 binds to the protein backbone of MCA, not to the large number of carbohydrate side chains. This antibody reacts with MCA producing cells.
MCA consists of a polymorphic family of glycoproteins. The b-12 related antigenic epitope is located in the more constant region of MCA. Specificity for Human MCA producing cells. |
| Storage | Aliquots of stock solution can be kept frozen at -70°C. Do not freeze working dilutions!! Avoid repeated freezing and thawing. Stability of stock solution: 1 year at -70°C. Shelf life: one year from despatch. |
| References | 1. Staehli, C. et al.: Monoclonal antibodies against antigens on breast cancer cells. Experientia 41: 1377. (1985)
2. Zenklusen, H.R. et al.: The immunohistochemical reactivity of a new anti-epithelial antibody (mAb b-12) against breast carcinoma and other normal and neoplastic human tissues. Virchows Arch A Pathol Anat 413: 3. (1988) 3. Maurer, A. & Burckhardt, J.: Biochemistry and molecular biology of MCA. Int. J. Biol. Markers 8: 108-112. (1993) |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain with Mayer's hemalum. |
| Pictures | Table 1. b-12 Reaction Pattern on Human Tissues. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4077 Mucin-like carcinoma antigen'.