BM4097 Interleukin-16 (IL-16) antibody
see related secondary antibodiessee all 14 Interleukin-16 products
0.2 mg / US$ 585
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Interleukin-16 (IL-16) G11/II
Synonyms
IL16,
Product Description
Mouse anti Interleukin-16 (IL-16) G11/II, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | G11/II |
| Catalog Number | BM4097 |
| Quantity | 0.2 mg |
| Price | US$ 585 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4097.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | The leukocyte chemotactic factor (LCF) or interleukin-16 is a proinflammatory cytokine and natural soluble ligand to the human CD4 molecule. LCF is produced by CD4+ and CD8+ T lymphocytes and is considered essential to the influx of CD4+ T lymphocytes and macrophages into an inflammatory lesion. LCF has been detected in lymphocytes and microglial cells of multiple sclerosis lesions A large neuronal precursor (NIL-16) has recently been identified which can be detected in the cytosol of neurons in the cerebellum and hippocampus. |
| Immunogen | Recombinant human IL-16 |
| Format | State: Liquid Ig fraction Purification: Affinity chromatography BufferSystem: Phosphate buffered saline pH 7.2 (PBS), no stabilizer and 0.09 % sodium azide as a preservative |
| Applications | Immunohistochemistry on paraffin sections (2.5 - 5 µg/ml; 1:200 - 1:400; microwave pretreatment in citrate buffer is recommended for antigen retrieval).
Suggested positive control: Human spleen. ELISA. Western blot. |
| Specificity | G11/II detects LCF, a homotetrameric glycoprotein of 56 kDa
molecular weight that is composed of four noncovalently linked peptides of 14 kDa. Species: Human. |
| Storage | Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. |
| References | Schluesener, H.J. et al.: Leukocyte chemotactic factor, a natural ligand to CD4, is expressed by lymphocytes and microglial cells of the MS plaque. J. Neurosci. Res. 44: 606 - 11 (1996).
Kurschner, C. & Yuzaki, M.: Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein. J. Neurosci. 19: 7770 - 80 (1999). |
| Protocols | Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature
1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7 min. at 700 Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4097 Interleukin-16 (IL-16)'.