BM4098 Endostatin (Collagen XVIII) antibody
see related secondary antibodiessee all 28 Endostatin products
0.2 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Endostatin (Collagen XVIII) 1837-46
Synonyms
,
Product review
Please read our product review about Endostatin (Collagen XVIII): Angiogenesis Research Antibodies.
Product Description
Mouse anti Endostatin (Collagen XVIII) 1837-46, Presentation: Purified. Product is tested for Frozen sections ( C ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | 1837-46 |
| Catalog Number | BM4098 |
| Quantity | 0.2 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4098.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | Endostatin is a powerful inhibitor of endothelial cell proliferation and angiogenesis and shows widespread distribution, mainly in vessel walls and some other basement membrane zones. |
| Immunogen | Recombinant human Endostatin (the carboxy-terminal domain)
|
| Format | State: Liquid IgG fraction Purification: Affinity chromatography BufferSystem: PBS, pH 7.2, containing 0.09% sodium azide as preservative |
| Applications | Immunohistochemistry on frozen and paraffin sections: 10 µg/ml (1:100).
Microwave pretreatment in citrate buffer is recommended for antigen retrieval in paraffin embedded tissues. Recommended positive control: Human tonsil. |
| Specificity | This antibody is directed against the Endostatin portion (the carboxy-terminal domain) of collagen XVIII. Species: Human. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots)at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | Strik H.M. et al.: Localization of endostatin in rat and human gliomas. Cancer 91: 1013-9 (2001)
|
| Protocols | Immunohistochemistry on paraffin sections:
1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water 2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0.01M citrate buffer pH 6.0. 3) Incubate slides for 15min in 200ml methanol/4.8ml 30%H2O2 4) Rinse slides 2x in distilled water and for 5min in TBS, pH 7.5 5) Block with normal swine serum (NSS, Jackson Immunoresearch #014-000-121) diluted 1:10 in Tris buffered saline pH 7.5, containing 0.1% bovine serum albumin (BSA, Calbiochem). Cover sections with NSS and incubate for 15min in a humid chamber at room temperature (RT). 6) Remove serum from sections (do not rinse) 7) Incubate with primary antibody diluted in TBS/0.1%BSA, pH 7.5 for 60min in a humid chamber at room temperature 8) Rinse sections in TBS, pH 7.5 for 5min, 2x 9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin, Dako E 0464) 1:400 diluted in TBS/0.1%BSA, pH 7.5 for 30min in a moist chamber, room temperature. Preincubate now StreptABComplex /HRPO in TBS/0.1%BSA, pH 7.5! 10) Rinse sections in TBS, pH 7.5 for 5min, 2x 11) Incubate with StreptABComplex /HRPO (DAKO, K 0377) for 30min in a humid chamber at room temperature 12) Rinse sections in TBS, pH 7.5 for 5min, 2x 13) DAB-staining Prepare 3,3 - diaminobenzidintetrahydrochloride (DAB) according the manufacturers instructions (e.g. DAB tablets) and incubate slides for 1-15 min. Check color reaction to avoid overstaining. 14) Rinse sections in TBS, pH 7.5 for 5min, 2x 15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min 16) Mount slides with coverslips |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4098 Endostatin (Collagen XVIII)'.