BM4108 Complement SC5b-9 (TCC / MAC) antibody
see related secondary antibodiessee all 3 Complement SC5b-9 products
0.2 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Complement SC5b-9 (TCC / MAC) X-11
Product Description
Mouse anti Complement SC5b-9 (TCC / MAC) X-11, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | X-11 |
| Catalog Number | BM4108 |
| Quantity | 0.2 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4108.pdf |
Datasheet Extract
| Concentration | 0.4 mg/ml |
| Background | Monoclonal antibody X-11 detects a neoepitope of the complement component C9 which is expressed when C9 is assembled in the terminal complement complex (TCC). Follicular dendritic cells and sinus lining cells in lymph node and tonsil, and blood dendritic cells express the X-11 antigen. X-11+ blood dendritic cells show typical dendritic veils and are the most potent stimulator cells in an allogenic mixed leukocyte reaction when compared with other leukocytes. X11+ cells strongly react with anti S100 and weakly with anti CD68. The literature suggests that X-11+ cells are dendritic cells of monocytic origin. |
| Immunogen | Enriched human blood dendritic cells. Remarks: The antigen is a neoepitope of C9 after assembly in the terminal complement complex. Epitope sequence has not been determined. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS buffer, pH 7.2 with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry (IHC); has been described to work in FACS.
Recommended Dilutions for IHC: Frozen sections: 0.5 μg/ml (1:800) Paraffin sections: 2 μg/ml (1:200); microwave pretreatment for antigen retrieval is recommended. |
| Specificity | Positive on dendritic cells in the peripheral
blood. It is absent from all other blood cells. LPS or IFN-γ stimulated monocytes do not express the antigen. The antigen is found on follicular dendritic cells of lymph nodes in the B-cell dependent areas. Some macrophages of the tonsil stain also positive with the antibody. It is absent from the thymus and the skin. In the spleen, trabeculae are stained positively by X-11. Species: Human: follicular dendritic (reticulum) cells; C9 neoepitope. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Halstensen, T.S. et al.: Terminal complement complex (TCC) and S-protein (vitronectin) on follicular dendritic cells in human lymphoid tissues. Immunology: 65, 193-197 (1988).
2. Zwirner, J. et al.: Complement activation in human lymphoid germinal centres. Immunolgy: 66, 270-277 (1989). 3. Würzner, R. et al.: Blood dendritic cells carry terminal complement complexes on their cell surface as detected by newly neoepitope-specific monoclonal antibodies. Immunolgy: 74, 132 - 138 (1991). 4. Peters, J.H. et al.: Differentiation of human monocytes into CD14 negative accessory cells: Do dendritic cells derive from the monocytic lineage. Pathobiology: 59, 122 - 126 (1991). 5.Tiemssen, C.T. et al.: Characterization of Human Blood Dendritic Cells: Cytokine Profiles. Abstr. 3rd Int. Symposium on Dendritic Cells in fundamental and Clinical Immunology, Annecy June 1994. |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4108 Complement SC5b-9 (TCC / MAC)'.