BM4111 CD68 / Macrosialin antibody
see related secondary antibodiessee all 46 CD68 / Macrosialin products
0.1 mg / US$ 525
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD68 / Macrosialin Ki-M7
Synonyms
CD68, Gp110, Macrosialin,
Product Description
Mouse anti CD68 / Macrosialin Ki-M7, Presentation: Purified. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | Ki-M7 |
| Catalog Number | BM4111 |
| Swiss Prot. No. | P34810 |
| Quantity | 0.1 mg |
| Price | US$ 525 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4111.pdf |
Datasheet Extract
| Immunogen | Human lymph node tissue. Swiss Num.: P34810 |
| Format | State: Lyophilised Ig fraction Purification: Affinity chromatography BufferSystem: Phosphate buffered saline pH 7.2 (PBS), 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.02 % sodium azide as a preservative Reconstitution: Restore in 0.5 ml distilled water to a concentration of 0.2 mg/ml. |
| Applications | Immunohistochemistry on frozen sections (1:800). Does not work in routinely processed paraffin sections.
Suggested positive control: Human tonsil. |
| Specificity | This antibody detects monocytes and macrophages.
The antigen is intracellular and yields a granular reaction pattern. It increases with monocyte differentiation into macrophages and has been clustered as CD68. The epitope has not been further characterized. Antigen distribution: Isolated cells: In human blood and bone marrow Ki-M7 remains restricted to monocytes and macrophages with a granular cytoplasmic reaction pattern. Tissue sections: Only macrophages reveal a positive reaction with the exception of follicle mantle lymphocytes also being positive for Ki-M7. Thus, some B-cell lymphoma also react with Ki-M7. Species: Human. |
| Storage | Store the lyophilized product at 2 - 8 °C.
Following reconstitution store in aliquots at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Kreipe, H. et al.: Ki-M7, Monoclonal Antibody Specific for Myelomonocytic Cell Lineage and Macrophages in Human. J. Histochem. Cytochem.: 35, (10), 1117 - 1126 (1987).
Eischen, A. et al.: Phenotypic characterization of human peritoneal and alveolar macrophages, and of human blood monocytes differentiated in the presence of GM-CSF or M-CSF. Fifth Annual Conference of the Upper Rhine Universities on the Macrophage, Strasbourg Sept. 4/5. Abstr. Page 22 (1991). Neumann, K.: Reactivity pattern of subtypes of macrophages in benign and malignant tumors. Fifth Annual Conference of the Upper Rhine Universities on the Macrophage, Strasbourg Sept. 4/5. Abstr. 74 (1991). Parwaresch, M.R.: Human Macrophage hybrid forming spontaneous giant cells. Virchows Arch.: 51, 89 - 86 (1986). |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1 h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1 h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4111 CD68 / Macrosialin'.
