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Staining of human peripheral blood monocytes with mouse anti human CD68: FITC (BM4111F) following permeabilisation

 

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BM4111F  CD68 / Macrosialin antibody

see related secondary antibodies
see all 46 CD68 / Macrosialin products
100 Tests / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD68 / Macrosialin Ki-M7

Synonyms

CD68, Gp110, Macrosialin,

Product Description

Mouse anti CD68 / Macrosialin Ki-M7, Presentation: FITC. Product is tested for Flow cytometry ( F )

Properties

ApplicationsFlow cytometry ( F )
ReactivityHuman ( Hu )
PresentationFITC
HostMouse
IsotypeIgG1
CloneKi-M7
Catalog NumberBM4111F
Swiss Prot. No.P34810
Quantity100 Tests
PriceUS$ 455
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
BM4111F.pdf

Datasheet Extract

Alternate namesMacrosialin
BackgroundElevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukaemia studies.
ImmunogenCell suspensions of human lymph node
Swiss Num.: P34810
Format
State: Liquid
Purification: Protein A purified from tissue culture supernatant
BufferSystem: PBS buffer pH 7.4 with 0.05 % Sodium Azide as preservative and 1 % Bovine Serum Albumin as stabiliser
Label: Fluorescein Isothiocyanate Isomer 1 (FITC)
Absorptionemission: 495 nm / 528 nm
ApplicationsFlow Cytometry (Membrane permeabilisation is required; Use 10µl to label 10e6 cells in 100 µl).
Clone KiM7 has been reported as being also suitable for use in Immunoprecipitation and Immunohistochemistry on frozen sections.
SpecificityBM4111F recognizes the 110KD integral membrane glycoprotein CD68 (Macrosialin) predominantly expressed on the intracellular lysosomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes.
Species: Human.
StorageStore the antibody undiluted at 2-8°C. DO NOT FEEZE. This product is photosensitive and should be protected from light. Shelf life: One year from despatch.
References1. Kreipe, H. et al. (1987). Ki-M7 Monoclonal Antibody Specific for Myelomonocytic Cell Lineage and Macrophages in Human. J. Histochem. Cytochem. 35(10): 1117-1126.
2. Diop, O.M. et al. (2000). High Levels of Viral Replication during Primary Simian Immunodeficiency Virus SIVagm Infection Are Rapidly and Strongly Controlled in African Green Monkeys. J. Virology 74(16): 7538-7547.
3. Micklem, K. et al. (1989). A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Brit. J. Haem 73: 6-11.
4. Glushakova, S.et al. (1999). Nef Enhances Human Immunodeficiency Virus Replication and Responsiveness to Interleukin-2 in Human Lymphoid Tissue Ex Vivo. J. Virology 73(5): 3968-3974.
5. Sadovnikova, E. et al. (2002). The CD68 protein as a potential target for leukaemia-reactive CTL. Leukemia 16(10): 2019-2026.
PicturesStaining of human peripheral blood monocytes with mouse anti human CD68: FITC (BM4111F) following permeabilisation

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 315
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 295
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 295
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 305
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 325
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 455
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 455
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 455
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 455
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 455
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 465
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 465
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 465
   

Click here to see all secondary antibodies for 'BM4111F CD68 / Macrosialin'.