BM4112 CD163 antibody
see related secondary antibodiessee all 19 CD163 products
0.1 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD163 Ki-M8
Synonyms
CD163, M130, Hemoglobin scavenger receptor, , Scavenger receptor cysteine-rich type 1 protein M130
Product Description
Mouse anti CD163 Ki-M8, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | Ki-M8 |
| Catalog Number | BM4112 |
| Swiss Prot. No. | Q86VB7 |
| Quantity | 0.1 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4112.pdf |
Datasheet Extract
| Alternate names | Scavenger Receptor, CD163 |
| Concentration | 0.2 mg/ml |
| Background | Human macrophages, monocytes in bone marrow and peripheral blood. Ki-M8 is negative with all other leucocytes. Ki-M8 detects an epitope of the cytoplasmic, lysosome-associated antigen CD163 which is increasingly expressed during cell differentiation. Parts of the outer cell membrane are also reported to be positive. Ki-M8 positive cells therefore show a granular staining pattern. In tissue sections, Ki-M8 detects phagocytic macrophages in most tissues. It is also positive with epithelial cells and malignant histiocytosis. Starry sky macrophages of lymphoids are negative.
Ki-M8 recognizes a membrane glycoprotein on human monocytes and macrophages which is expressed in the intermediate and late phase of inflammation. CD163 is a scavenger receptor for the hemoglobin-haptoglobin complex, and is significantly upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions. |
| Immunogen | Human alveolar macrophages Swiss Num.: Q86VB7 |
| Format | State: Lyophilized purified Ig fraction Purification: Protein A chromatography BufferSystem: PBS buffer pH 7.2 with 0.02 % sodium azide as preservative and 10 mg/ml BSA as stabilizer. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections: 2 µg/ml (1:100), FACS and Western blots.
Positive control: human tonsil. |
| Specificity | Ki-M8 detects phagocytic macrophages in most tissues. It is also positive with epithelial cells and malignant histiocytosis.
Starry sky macrophages of lymphoids are negative. Species: Human. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Radzun, H.J. et al.: Ki-M8 Monoclonal Antibody Reactive with an Intracytoplasmic Antigen of Monocyte/macrophage Lineage. Blood: 69, (5), 1320 - 1327 (1987).
2. Kiemle-Kalle, J. et al.: Alveolar macrophages in idiopathic pulmonary fibrosis display a more monocyte-like immunophenotype and an increased release of free oxygen radicals. Eur. Respir J.: 4, 400 - 406 (1991). 3. Parwaresch, M.R. et al.: Human Macrophage hybrid forming spontaneous giant cells. Virchows Arch.: 51, 89 - 86 (1986). 4. Parwaresch, M.R. et al.: M-CSF and M-CSF-Receptor Gene Expression in acute Myelomonocytic Leukemias. Leukemia Res.: 14, (1), 27 - 37 (1990). 5. Radzun, H.J. et al.: Modulation of c-fms Proto-Oncogene Expression in Human Blood Monocytes and Macrophages. J. of Leukocyte Biol.: 44, 198 - 204 (1988). 6. Neumann, K.: Reactivity pattern of subtypes of macrophages in benign and malignant tumors. Fifth Annual Conference of the Upper Rhine Universities on the Macrophage, Strasbourg Sept. 4/5. Abstr. 74 (1991). 7. Eischen, A. et al.: Phenotypic characterization of human peritoneal and alveolar macrophages, and of human blood monocytes differentiated in the presence of GM-CSF or M-CSF. Fifth Annual Conference of the Upper Rhine Universities on the Macrophage, Strasbourg Sept. 4/5. Abstr. Page 22 (1991). |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4112 CD163'.
