BM4113 Angiogenin antibody
see related secondary antibodiessee all 9 Angiogenin products
0.15 mg / US$ 505
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Angiogenin MANG1
Synonyms
,
Product review
Please read our product review about Angiogenin: Angiogenesis Research Antibodies.
Product Description
Mouse anti Angiogenin MANG1, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgM |
| Clone | MANG1 |
| Catalog Number | BM4113 |
| Quantity | 0.15 mg |
| Price | US$ 505 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4113.pdf |
Datasheet Extract
| Alternate names | Ribonuclease 5 |
| Concentration | 0.3 mg/ml |
| Background | Monoclonal antibody MANG-1 is useful for detecting human angiogenin, a potent vessel inducing basic polypeptide, with a unique ribonucleolytic activity. |
| Immunogen | Recombinant human Angiogenin (Bachem Feinchemikalien AG). |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS, pH 7.2 with 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry and ELISA.
Recommended Dilutions for IHC: Frozen sections: 5 µg/ml (1:60) Paraffin sections: 10 µg/ml (1:30). Proteinase K pretreatment for antigen retrieval is recommended. |
| Specificity | Antigen Distribution on tissue sections:
MANG-1 stains single cells on cryostat sections of human tonsil. In human terminal placenta endothelial cells are stained positive. Angiogenin is a 14.1kD single chain polypeptide of 123 amino acids. MANG-1 is positive on recombinant angiogenin in ELISA. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. FETT, J.W., STYRDOM, D., LOBB, R.,ALDERMAN, E., BETHUNE, L., RIODAN, J., VALLEE, B: Isolation and Characterization of Angiogenin, an angiogenic Protein from Human Carcinoma Cells. Biochemistry: 24, (1985) 5480-5486.
2. SHAPIRO, R. & VALLEE, B.L.: Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin. Proc. Natl. Acad. Sci. 84, (1987): 2238-2241. 3. KURACHI, K., DAVIE, E.W., STRYDOM, D.J., RIORDAN, J.F., VALLEE, B.L.: Sequence of the cDNA and Gene for Angiogenin, a human Angiogenesis Factor. Biochemistry: 24 (1985): 5494-5499. |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain with Mayer's hemalum |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 365 | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 345 | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 345 | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 365 | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 375 | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / US$ 395 | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / US$ 374 | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / US$ 474 | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / US$ 374 | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / US$ 425 | ||||
Click here to see all secondary antibodies for 'BM4113 Angiogenin'.