BM4118 Macrophages (Marginal Metallophils) antibody
see related secondary antibodiessee all 59 Macrophages products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages (Marginal Metallophils) TRPM-3
Synonyms
Rat Macrophage Marker, ,
Product review
Please read our product review about Macrophages (Marginal Metallophils): Antibodies to Rat Macrophages.
Product Description
Mouse anti Macrophages (Marginal Metallophils) TRPM-3, Presentation: Purified. Product is tested for Frozen sections ( C )
Properties
| Applications | Frozen sections ( C ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | TRPM-3 |
| Catalog Number | BM4118 |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4118.pdf |
Datasheet Extract
| Alternate names | Marginal Metallophils |
| Concentration | 0.2 mg/ml |
| Background | TRPM-3 is a specific cell surface marker useful for the identification of tissue fixed rat macrophages and macrophage subpopulations. It is particularly suitable for phenotyping macrophages of lymphoid organs, where it reacts with marginal zone macrophages and with marginal metallophils. It is also an interesting marker for studying autoimmune diseased tissues.
TRPM-3 recognises an epitope of a membrane protein which is associated with a receptor for glycoconjugates (sialoadhesin). |
| Immunogen | Peritoneal macrophages. |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS, pH 7.2, with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Suitable for Immunohistochemistry on frozen sections:
0.25-0.5 µg/ml (1/400-1/800) when using an ABC amplification system. |
| Specificity | Reacts with rat macrophages.
Negative in human, mouse, hamster, guinea pig , goat, rabbit. TRPM-3 is negative with monocytes and granulocytes. TRPM-3 stains marginal zone macrophages and marginal metallophils of the spleen strongly. It rarely reacts with cordal macrophages in the red pulp and interdigitating cells in the periarterial lymphatic sheet of the spleen. In lymph nodes, only sinus macrophages express the antigen. Moreover, it is expressed on macrophages in the synovial membrane, mucosa and submucosa of the gastrointestinal tract, twisted spindle-shaped macrophages of the omentum and on macrophages in the dermis and subcutaneous tissue. It is absent from macrophages in the thymus, Peyer's patches and from Langerhans cells in the skin. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Takeya,M., L. Hsiao, K. Takahashi: A new monoclonal antibody, TRPM-3, binds specifically to certain rat macrophage populations: Immunohistochemical and immunoelectron microscopic analysis. J Leukocyte Biol 41: 187-195. (1987)
2. T.Kato, M.Takeya, K. Takagi, K. Takahashi: Chemical induced Transplantable malignant fibrous histiocytoma of the rat. Lab Invest 62: 635-645. (1990) 3. S. Izumi, M Takeya, K. Takagi, K. Takahashi. Ontogenic development of synovial A cells in fetal and neonatal rat knee joints. Cell Tis Res 262: 1-8. (1990) 4. Y. Yamaguchi et al. Effect of Cyclosporine on Distribution of macrophage subpopulations in rat hepatic allograft. Dig Disease and Sci. 38: 619-625. (1993) |
| Pictures | Figure 1. Immunohistochemistry on frozen sections using monoclonal antibody BM4118. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4118 Macrophages (Marginal Metallophils)'.
