BM4502 Lamin B2 antibody
see related secondary antibodiessee all 4 Lamin B2 products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Lamin B2 LN43
Synonyms
filament-type protein,
Product Description
Mouse anti Lamin B2 LN43, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Hamster ( Hst ), Xenopus ( Xen ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | LN43 |
| Catalog Number | BM4502 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4502.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and amin B2. |
| Immunogen | Detergent insoluble fraction of potoroo cell line PtK1. |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS with 0.09% Sodium Azide as preservative. |
| Applications | Suitable for Immunocytochemistry on frozen sections, Immunoblotting and Flow cytometry.
Recommended dilutions: 1/100-1/200 for Flow cytometry and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. 1/100-1/1000 for Immunoblotting applications. |
| Specificity | Reacts with an epitope located in the C-terminal part of lamin B2.
Recognises lamin B2 in human, mouse, hamster and Xenopus. |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch. |
| References | 1. Bridger, J. M., Kill, I. R., O'Farrell, M., and Hutchison, C. J. (1993). Internal lamin structures within G1 nuclei of human dermal fibroblasts, J Cell Sci 104, 297-306.
2. Jenkins, H., Hölman, T., Lyon, C., Lane, B., Stick, R., and Hutchison, C. J. (1993). Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix, J Cell Sci 106, 275-285. 3. Hozak, P., Sasseville, A. M., Raymond, Y., and Cook, P. R. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells, J Cell Sci 108, 635-44. 4. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. 5. Machiels, B. M., Zorenc, A. H., Endert, J. M., Kuijpers, H. J., van Eys, G. J., Ramaekers, F. C., and Broers, J. L. (1996). An alternative splicing product of the lamin A/C gene lacks exon 10, J Biol Chem 271, 9249-53. 6. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. 7. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. 8. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal human tissues, Histochem Cell Biol 107, 505-17. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4502 Lamin B2'.
