BM4534 RAP1 antibody
see related secondary antibodiessee all 17 RAP1 products
50 µl / US$ 445
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti RAP1 5G7/1
Product Description
Mouse anti RAP1 5G7/1, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 5G7/1 |
| Catalog Number | BM4534 |
| Quantity | 50 µl |
| Price | US$ 445 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4534.pdf |
Datasheet Extract
| Alternate names | TERF2IP, TRF2 Interacting Telomeric Protein RAP1, Telomeric Repeat Binding Factor 2 Interacting Protein
|
| Background | Rap1 is located at telomeres and affects telomere length. However, unlike yeast Rap1 that binds telomeric DNA directly, human Rap1 is recruited to telomeres by TRF2. |
| Immunogen | Human Rap1, MTA19. |
| Format | State: Liquid purified Ig fraction. Purification: Protein A chromatography. BufferSystem: PBS, pH 7.2 with 0.09% sodium azide as preservative. |
| Applications | Suitable for Elisa and Western blot (1/100-1/200). |
| Specificity | Human Rap1. |
| Storage | Store the antibody at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| Protocols | Western Blotting Protocol
1. Transfer gel to PDVF or nitrocellulose membrane 2. Place membrane in plastic tray in blocking buffer for one hour with agitation 3. Rinse in wash buffer 4. Incubate in wash buffer plus primary antibody for one hour 5. Wash 6 X 5 minutes with wash buffer 6. Incubate in wash buffer plus secondary antibody for one hour 7. Wash 6X 5 minutes with wash buffer 8. Detect (e.g. ECL, Amersham according to manufacturers instructions). Wash buffer: PBS + 0.1% Tween 20 Blocking buffer: Wash buffer + 5% dried milk powder. The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM4534 RAP1'.