BM5010 Adeno-Associated Virus 2 (intact particle) antibody
see related secondary antibodiessee all 8 Adeno-Associated Virus 2 products
50 µg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Adeno-Associated Virus 2 (intact particle) A20
Synonyms
AAV2,
Product Description
Mouse anti Adeno-Associated Virus 2 (intact particle) A20, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Functional assay ( FN ), Immunofluorescence ( IF ), Immunoprecipitation ( IP )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Functional assay ( FN ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG3 |
| Clone | A20 |
| Catalog Number | BM5010 |
| Quantity | 50 µg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5010.pdf |
Datasheet Extract
| Immunogen | Adeno-associated virus capsid proteins and virus particles |
| Format | State: Lyophilized Purification: Protein A affinity chromatography BufferSystem: PBS or Tris-buffered saline contains 0.09% sodium azide and 0.5% BSA Reconstitution: Restore with 1 ml dist. water |
| Applications | Immunofluorescence microscopy: 1:20; Overnight at 2-8°C.
Immunohistochemistry: Overnight at 2-8°C. Immunoprecipitation: 1:5. Neutralization assay. Affinity chromatography. ELISA. |
| Specificity | For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting. Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV-2 capsids. The antibody is also useful for neutralizing experiments (cf. Moskalenko et.al). |
| Storage | Store the antibody undiluted at 2-8°C.
DO NOT FREEZE! Shelf life: one year from despatch. |
| References | 1. Wistuba A, Weger S, Kern A, and Kleinschmidt J: Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J Virol 69, 5311-5319 (1995).
2. Wistuba A, Kern A, Weger S., Grimm D, and Kleinschmidt J: Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol 71, 134-1352 (1997). 3. Grimm D, Kern A, Pawlita M, Ferrari F, Samulski R, Kleinschmidt J: Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Ther 6(7) 1322-1330 (1999). 4. Bartlett JS, Kleinschmidt J, Boucher RC, and Samulski RJ: Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(abâ??γ)2 antibody. Nature Biotechnology 17, 181-186 (1999) 5. Wobus C A, Hügle-Dörr B, Girod A, Petersen G, Hallek M and Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virology 74, 9281-9293 (2000). 6. Moskalenko M, Chen L, van Roey M, Donahue BA, Snyder RO, McArthur JG, Patel SD: Epitope mapping of human anti-adenoassociated virus type 2 neutralizing antibodies: Implications for gene therapy and virus structure. J Virology 74, 1761-1766 (2000). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM5010 Adeno-Associated Virus 2 (intact particle)'.
